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Adipokinetic hormone enhances nodule formation and phenoloxidase activation in adult locusts injected with bacterial lipopolysaccharide

By Graham J. Goldsworthy, S. Chandrakant and K. Opoku-Ware


Interactions between the locust endocrine and immune systems have been studied in vivo in relation to nodule formation and activation of the prophenoloxidase cascade in the haemolymph. Injection of bacterial lipopolysaccharide (LPS) extracted from Escherichia coli induces nodule formation in larval and adult locusts but does not increase phenoloxidase activity in the haemolymph. Nodule formation starts rapidly after injection of LPS and is virtually complete within 8 h, nodules occurring mainly associated with the dorsal diaphragm on either side of the heart, but sometimes with smaller numbers associated with the ventral diaphragm on either side of the nerve cord. Co-injection of adipokinetic hormone-I (Lom-AKH-I) with LPS stimulates greater numbers of nodules to be formed in larval and adult locusts, and activates phenoloxidase in the haemolymph of mature adults but not of nymphs. The effect of co-injection of Lom-AKH-I with LPS on nodule formation is seen at low doses of hormone; only 0.4 pmol of Lom-AKH-I per adult locust is needed to produce a 50% increase in the number of nodules formed. When different components of LPS from the E. coli Rd mutant are tested, the mono- and the diphosphoryl Lipid A components have similar effects to the intact LPS. Remarkably, detoxified LPS activates phenoloxidase in the absence of Lom-AKH-I, although co-injection with hormone does enhance this response. Both diphosphoryl Lipid A and detoxified LPS induce a level of nodule formation that is enhanced by co-injection of Lom-AKH-I, but monophosphoryl Lipid A does not initiate nodule formation even when injected with hormone. Co-injection of a water-soluble inhibitor of eicosanoid synthesis, diclofenac (2-[(2, 6-dichlorophenyl)amino] benzeneacetic acid), reduces nodule formation in response to injections of LPS (both in the absence and presence of hormone) in a dose-dependent manner, but does not prevent activation of phenoloxidase in adult locusts. It is shown that nodule formation and activation of the prophenoloxidase in locust haemolymph can both be enhanced by Lom-AKH–I, but it is argued that these processes involve distinct mechanisms in which eicosanoid synthesis is important for nodule formation, but not for the increased phenoloxidase activity

Topics: bcs
Publisher: Elsevier
Year: 2003
OAI identifier: oai:eprints.bbk.ac.uk.oai2:240

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  1. (1998). A s h i d a doi
  2. (1991). Activati o n of the propheo l o x i d a s e cascade and in itiati o n of nodule for matio n in locust s by bacter i a l lipopo l y s a c c h a r i d e s . Develo p me n t a l and Compa r a t i v e Immun o l o g y 15,
  3. (1991). Activation of the propheoloxidase cascade and initiation of nodule formation in locusts by bacterial lipopolysaccharides. doi
  4. (2002). Adipokinetic hormone enhanc e s lami na r i n and bacter i a l lip op o l y s a c c ha r i d e - i n d u c e d activa t i o n of the prophe n o l o x i d a s e cascad e in the Africa n migrat o r y locust , Locusta migratoria.
  5. (2002). Adipokinetic hormone enhances laminarin and bacterial lipopolysaccharide-induced activation of the prophenoloxidase cascade in the African migratory locust, Locusta migratoria. doi
  6. Ag e relate d change s in the respon s e to adipok i n e t i c hormon e in Locusta migratoria. Physio l o g i c a l Entomo l o g y 2,
  7. (1977). Age related changes in the response to adipokinetic hormone in Locusta migratoria. doi
  8. (2002). Binding of hemolin to bacterial lipopolysaccharide and lipoteichoic acid - An immunoglobulin superfamily member from insects as a pattern-recognition receptor. doi
  9. (1996). Biochemi s t r y and cell biolog y of bacter i a l endoto x i n s .
  10. (1996). Biochemistry and cell biology of bacterial endotoxins. doi
  11. (1997). Biological mediators of insect immunity. doi
  12. (1973). Blood for ming tissues in orthopte r a n insects, an analogue to verteb r a t e hemopo i e t i c or gans. Experie n t i a
  13. (1973). Blood forming tissues in orthopteran insects, an analogue to vertebrate hemopoietic organs. doi
  14. Di glyc e r i d e transp o r t i n g lipopr o t e i n s in Locusta .
  15. (1977). Diglyceride transporting lipoproteins in Locusta. doi
  16. Ed wards 1999. Parasiti z a t i o n of Lacanobia oleracea (Lepido p t e r a , Noctuidae) by the ectoparasite wasp, Eulophus pennicornis - Effects of parasit i z a t i o n , venom and starvat i o n on host haemocy t e s .
  17. Edwards 1999. Parasitization of Lacanobia oleracea (Lepidoptera , Noctuidae) by the ectoparasite wasp, Eulophus pennicornis - Effects of parasitization, venom and starvation on host haemocytes. doi
  18. (1999). Eicosa n o i d s mediat e nodula t i o n reacti o n s to bacteri a l in fections in adults of the cricket, Gryllus assimilis .
  19. (2001). Eicosano i d s mediat e microa g g r e g a t i o n reacti o n s to bacter i al chall e n g e in isol a t e d insect he mocy t e prepar a t i o n s . Journa l of Insect Physiol o g y
  20. (1996). Eicosanoids mediate insect nodule responses to bacterial infections in larvae of the tenebrionid, Zophobas atratus. doi
  21. (1994). Eicosanoids mediate insect nodule responses to bacterial infections. doi
  22. (2001). Eicosanoids mediate microaggregation reactions to bacterial challenge in isolated insect hemocyte preparations. doi
  23. (1999). Eicosanoids mediate nodulation reactions to bacterial infections in adults of the cricket, Gryllus assimilis. doi
  24. (1975). Goldsw o r t h y
  25. (1988). Haemocyte behaviour. A dvances in doi
  26. (1985). He mocytic aggregation in Schistocerca gregaria and Periplaneta americana as a respon s e to in jected substances of microbial origin.
  27. (1985). Hemocytic aggregation in Schistocerca gregaria and Periplaneta americana as a response to injected substances of microbial origin. doi
  28. (1997). Immune responses in Rhodnius prolixus , influence of nutrition and ecdys one . doi
  29. (1997). Immune responses in Rhodnius prolixus, influence of nutrition and ecdysone. doi
  30. (2000). Insect cellular reactions to the lipopolysaccharide component of the bacterium Serratia marcescens are mediated by eicosanoids. doi
  31. (2002). Insect haemoc y t e s and their role in immunity. Insect Biochemi s t r y and Molecul a r
  32. (2002). Insect haemocytes and their role in immunity. doi
  33. (1991). Insect immu ne respon s e to bacteri a l infect i o n is mediate d by eicosan o i d s .
  34. (1991). Insect immune response to bacterial infection is mediated by eicosanoids. doi
  35. (1985). Inverteb r a t e Immunity - Basic Concepts and Recent A dvances . Internat i o n a l Review of Cytology 97, doi
  36. (1985). Invertebrate Immunity - Basic Concepts and Recent Advances. doi
  37. (1970). Les organe s haema t o p o i t i qu e de deux insect e s ortho p t e r e s , Locusta migratoria et Gryllus bimaculatus. Zeitschr i f t fur Zellfors c h u n g und Mikroskopische Anatomie.
  38. (1970). Les organes haematopoitique de deux insectes orthopteres, Locusta migratoria et Gryllus bimaculatus. Zeitschrift fur Zellforschung und Mikroskopische Anatomie. doi
  39. (1997). Lipopo l y s a c c h a r i d e - b i n d i n g prot ei n s and their involv e me n t in the bacteri a l cleara n c e from the hemoly mp h of the silkwo r m Bombyx mori.
  40. (1997). Lipopol y s a c c h a r i d e intera c t i o n with hemoli n , an insect me mber of the Ig-supe r f a mi l y .
  41. (1997). Lipopolysaccharide interaction with hemolin, an insect member of the Ig-superfamily. doi
  42. (1997). Lipopolysaccharide-binding proteins and their involvement in the bacterial clearance from the hemolymph of the silkworm Bombyx mori. doi
  43. (2002). Modulation by eicosanoid biosynthesi s inhibito r s of immune response s by the insec t Manduca sexta to the pathoge n i c fungus Metarhizium anisopliae . Journ a l of Invertebrate Pathology doi
  44. (2002). Modulation by eicosanoid biosynthesis inhibitors of immune responses by the insect Manduca sexta to the pathogenic fungus Metarhizium anisopliae. doi
  45. (1959). Observa t i o n s on a probabl e blood for min g (leuco p o i e t i c ) tissue
  46. (1959). Observations on a probable blood forming (leucopoietic) tissue in Locusta migratoria phase danica (Orthoptera,
  47. (1994). Oct opa mi n e , a modula t o r of the hemocy t i c nodulati o n response of non-immu n e Galleria mellonella larvae. Journa l of Insect Physio l o g y 40,
  48. (1992). Octopa mi n e and 5-hydr o x y t r y p t a mi n e enhanc e the phagoc y t i c and nodule form at i o n activi t i e s of cockro a c h ( Periplaneta americana) hemoc y t e s . Journ a l of Insect
  49. (1992). Octopamine and 5-hydroxytryptamine enhance the phagocytic and nodule formation activities of cockroach (Periplaneta americana) hemocytes. doi
  50. (1994). Octopamine, a modulator of the hemocytic nodulation response of non-immune Galleria mellonella larvae. doi
  51. Proceed i n g s of the Nationa l
  52. (1998). Recent advances in research on the insect phenoloxidase cascade. In: Molecular mechanisms of immune responses in insects
  53. (1994). S a m u e l s o n D.W. doi
  54. (2000). St anl e y
  55. (1996). StanleyS a mu e l s o n
  56. (1975). Starvation and haemolymph lipids in Locusta migratoria migratorioides
  57. (1997). Structur e s , assay s and recep t or s for locus t adipoki n e t i c hormon e s . Comp ar a t i v e Bioche mi s t r y and Physiology B.
  58. (1997). Structures, assays and receptors for locust adipokinetic hormones. doi
  59. (1989). Studie s on nodule format i o n in locust s follow i n g inject i o n of microb i a l product s . Journal of Inverte b r a t e Patholo g y 53,
  60. (1989). Studies on nodule formation in locusts following injection of microbial products. doi
  61. (1989). Studies on the activati o n of the prophen o l o x i d a s e system of insects by b acteri a l - c e l l wall compone n t s . Insect Bioche mi s t r y 19,
  62. (1989). Studies on the activation of the prophenoloxidase system of insects by bacterial-cell wall components. doi
  63. (1985). The effects of diclof e n a c sodiu m on arachi d o n i c - a c i d metabol i s m. Se mi na r s in Arthri t i s and Rheuma t i s m
  64. (1985). The effects of diclofenac sodium on arachidonic-acid metabolism. doi
  65. (1999). The lipopolysaccharide-binding protein participating in hemocyte nodule formation in the silkworm Bombyx mori is a novel member of the C-type lectin superfamily with two different tandem carbohydrate-recognition domains. doi
  66. (1997). Trencz e k , T.,

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