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Changes in lipophorins are related to the activation of phenoloxidase in the haemolymph of Locusta migratoria in response to injection of immunogens

By Lisa M. Mullen and Graham J. Goldsworthy

Abstract

In Locusta migratoria, activation of phenoloxidase in the haemolymph in response to injection of laminarin is age-dependent: being absent in fifth instar nymphs and newly emerged adults, and only becoming evident four days after the final moult. This pattern of change in phenoloxidase activation correlates with the pattern of change in the concentration of apolipophorin-III (apoLp-III) in the haemolymph. Injection of a conspecific adipokinetic hormone (Lom-AKH-I) has no effect on the phenoloxidase response in nymphs or newly emerged adults but, in adults older than four days, co-injection of the hormone with laminarin prolongs the activation of phenoloxidase in the haemolymph: a similar enhancement of the response to laminarin is observed in locusts that have been starved for 48 h but not injected with AKH-I. During most of the fifth stadium, injection of laminarin results in a decrease in the level of prophenoloxidase in the haemolymph; an effect that is not observed in adults of any age. Marked changes in the concentration of apoLp-III, and the formation of LDLp in the haemolymph, are observed after injection of laminarin (or LPS) and these are remarkably similar, at least qualitatively, to those that occur after injection of AKH-I. The involvement of lipophorins in the activation of locust prophenoloxidase in response to immunogens is discussed

Topics: bcs
Publisher: Elsevier
Year: 2003
OAI identifier: oai:eprints.bbk.ac.uk.oai2:247

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  1. (1996). A n c s i n doi
  2. (2003). a nd Opoku-Wa r e , K. doi
  3. (1973). A si mpli f i e d metho d for the quanti t a t i v e assay of small amounts of protein in biologi c a l materi a l s . Analyt i c a l Bioche mi s t r y 51,
  4. (1973). A simplified method for the quantitative assay of small amounts of protein in biological materials. doi
  5. (2002). Adipokinetic hormone con cent r a t i o n s in the haemoly m p h of Schistocerca gregaria, measur e d by radioi m mu n o a s s a y . Insect Biochemi s t r y and Molecul a r
  6. (2002). Adipokinetic hormone concentrations in the haemolymph of Schistocerca gregaria, measured by radioimmunoassay. doi
  7. (2003). Adipokinetic hormone enhances nodule formation and phenoloxidase activation in adult locusts injected with bacterial lipopolysaccharide. doi
  8. (1977). Ag e-rel a t e d change s in the respon s e to adipok i n e t i c hormon e in Locusta migratoria. Physio l o g i c a l Entomo l o g y 2,
  9. (1977). Age-related changes in the response to adipokinetic hormone in Locusta migratoria. doi
  10. (2003). and Chandrak a n t , S.
  11. (1999). Apolip o p h o r i n - I I I in Galleria mellonella potent i a t es hemol y mp h lytic activi t y . Devel o p me n t a l and Compa r a t i v e
  12. Apolipo p h o r i n III levels in Locusta migratoria. Develo p me n t a l regula t i o n of gene express i o n and hemolymp h protei n concentration. Jo urnal of Insect Physiol o g y 42,
  13. Apolipophorin III levels in Locusta migratoria. Developmental regulation of gene expression and hemolymph protein concentration. doi
  14. (1999). Apolipophorin-III in Galleria mellonella potentiates hemolymph lytic activity. doi
  15. (1997). Biological mediators of insect immunity. doi
  16. (1994). Clearance of lipopolysaccharide in hemolymph of the Silkworm Bombyxmori - its role in the termination of cecropin messenger-RNA Induction. doi
  17. (2001). D e t t l o f f
  18. (1977). Di glyce r i d e - t r a n s p o r t i n g lipopr o t e i n s in Locusta .
  19. (1991). Different isof or ms of an apopro t e i n (apoli p o p h o r i n III) associat e with lipoprot e i n s in Locusta migratoria. European Journal of Bioche mi s t r y 196,
  20. (1991). Different isoforms of an apoprotein (apolipophorin III) associate with lipoproteins in Locusta migratoria. doi
  21. (1977). Diglyceride-transporting lipoproteins in Locusta. doi
  22. (1972). G o l d s w o r t h y doi
  23. (1976). Golds wor t h y , G.J.,
  24. (1997). Haem ol y mp h protei n s of larvae of Galleria mellonella detoxify endotoxins of the insect pathoge n i c bact e ri a Xenorhabdus nematophilus (Entero ba c t e r i a c e a e ) . doi
  25. (1997). Haemolymph proteins of larvae of Galleria mellonella detoxify endotoxins of the insect pathogenic bacteria Xenorhabdus nematophilus (Enterobacteriaceae). doi
  26. (1994). Hirama t s
  27. (1972). In vivo studies on the release of hormones from the corpora cardiaca of locusts. doi
  28. Infectio n and doi
  29. (1996). Insect hemocy t e adhesi o n in vitro: Inhibi t i o n by apolip h o r i n I and an artificial substr at e . Journal of Insect Physio l o g y 42,
  30. (1996). Insect hemocyte adhesion in vitro: Inhibition by apoliphorin I and an artificial substrate. doi
  31. (2001). Insect immune activati o n by apolipop h o r i n III is correla ted with the lipid-binding properties of this protein . Bioche mi s t r y
  32. (2001). Insect immune activation by apolipophorin III is correlated with the lipid-binding properties of this protein. doi
  33. (2002). Interac t i o n s between the endocri n e and i mmune systems in locusts . Physio l o g i c a l Entomol o g y 28: (in press) Goldswo r t h y , G.J., Opoku-Wa r e
  34. (2002). Interactions between the endocrine and immune systems in locusts. Physiological Entomology 28: (in press) doi
  35. (1997). Isolated apolip o p h or i n III from Galleria mellonella stimulate s the immune reaction s of this insect. doi
  36. (1997). Isolated apolipophorin III from Galleria mellonella stimulates the immune reactions of this insect. doi
  37. (1992). Lipophorin inhibits the adhesion of cockroach ( Periplaneta americana) hemocyt e s in vitro . doi
  38. (1992). Lipophorin inhibits the adhesion of cockroach (Periplaneta americana) hemocytes in vitro. doi
  39. (2001). Lipophorin of lower density is formed during immune responses in the lepidopteran insect Galleria mellonella. doi
  40. (1994). Lipopolysaccharide-lipophorin complex-formation in insect hemolymph - a common pathway of lipopolysaccharide detoxification both in insects and in mammals. doi
  41. (1985). Lipoprot e i n transfor ma t i o n s during adipokin e t i c hormone action in Locusta migratoria. Physiological Entomol o g y 10,
  42. (1985). Lipoprotein transformations during adipokinetic hormone action in Locusta migratoria. doi
  43. (2001). Localiz a t i o n of injecte d apolipo p h o r i n III in vivo - new insig h t s into the immun e activ a t i o n proces s direct e d by this protei n .
  44. (2001). Localization of injected apolipophorin III in vivo - new insights into the immune activation process directed by this protein. doi
  45. (2000). Molecul a r basis of exchan g e a b l e apolip o p r o t e i n functi o n . Biochi mi c a et Biophy s i c a Acta (BBA) - Molecu l a r and Cell Biology of Lipids
  46. (1995). Molecular analysis of hemoly mp h - p r o t e i n s - serpin Genes. Journa l of Cellul a r Bioche mi s t r y ,
  47. (1995). Molecular analysis of hemolymph-proteins - serpin Genes.
  48. (2000). Molecular basis of exchangeable apolipoprotein function. doi
  49. (1986). Physical and surface proper t i e s of insect apoli p o p h o r i n III. The Journal of Biologi c a l Che mistr y 261,
  50. (1986). Physical and surface properties of insect apolipophorin III.
  51. (1983). Pr otein- l i p o p r o t e i n interac t i o n s in the haemo l y mp h of Locusta during the action of adipokin e t i c hormone : the role of CL-prote i n s .
  52. (1983). Protein-lipoprotein interactions in the haemolymph of Locusta during the action of adipokinetic hormone: the role of CL-proteins. doi
  53. (1996). Purification and characterization of two storage proteins from Locusta migratoria showing distinct developmental and hormonal regulation. doi
  54. (1983). Qualitat i v e and quantit a t i v e changes in Locusta haemolymp h protein s and lipopro t e i n s during ageing and adipokin e t i c hormon e action . Journa l of In sect Physiolo g y 29,
  55. (1983). Qualitative and quantitative changes in Locusta haemolymph proteins and lipoproteins during ageing and adipokinetic hormone action. doi
  56. (1976). Quantitative studies on the release of locust adipokinetic hormone. Physiological Entomology, doi
  57. (1995). Role for circula t i n g lipopro t e i n s in protect i o n from endotox i n toxici t y .
  58. (1995). Role for circulating lipoproteins in protection from endotoxin toxicity.
  59. (1994). Shimabu k u r o
  60. (1972). Studies on insect adipo k i n e t i c hormo n e s . Gener a l a nd Compara t i v e Endocr i n o l o g y 18,
  61. (1972). Studies on insect adipokinetic hormones. doi
  62. (1983). The endocrin e contro l of flight meta b o l i s m in locust s .
  63. (1983). The endocrine control of flight metabolism in locusts. doi
  64. (1997). Trencz e k , T.,
  65. (1998). Two major prote i n s from locus t plasma are invol v e d in coagula t i o n and are specifi c a l l y preci pi t a t e d by lami nar i n , a beta-1, 3 - g l u c a n . Insect Bioche mi s t r y and Molecul a r Biolog y 28,
  66. (1998). Two major proteins from locust plasma are involved in coagulation and are specifically precipitated by laminarin, a beta-1,3-glucan. Insect Biochemistry and Molecular doi
  67. (1996). VanDerHo r s t ,

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