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Adipokinetic hormone enhances laminarin and bacterial lipopolysaccharide-induced activation of the prophenoloxidase cascade in the African migratory locust, Locusta migratoria

By Graham J. Goldsworthy, K. Opoku-Ware and Lisa M. Mullen


Lom-AKH-I enhances the activation in vivo of prophenoloxidase in the haemolymph of the African migratory locust, Locusta migratoria, in response to challenge with laminarin. AKH does not influence the speed or initial magnitude of the phenoloxidase response to laminarin, but prolongs the period of activation of the enzyme in a dose-dependent manner. Injections of preparations of bacterial lipopolysaccharide (LPS) do not activate prophenoloxidase in vivo, but co-injection of Lom-AKH-I with commercial preparations of LPS from Klebsiella pneumoniae, Escherichia coli, or Shigella flexneri (but not one from Pseudomonas aeroginosa) results in dose-dependent increases in the levels of phenoloxidase that persist in the haemolymph for several hours. It is argued that the effects of AKH on phenoloxidase activation in locusts described here are, at least in part, related directly to changes in lipid metabolism brought about by the hormone

Topics: bcs
Publisher: Elsevier
Year: 2002
OAI identifier:

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  1. (1991). Activati o n of the Prophe n o l o x i d a s e Casca d e and Initia t i o n of Nodule Forma t i o n in Locust s by Bacte r i a l Lipop o l y s a c c h a r i d e s . Devel o p me n t a l and Compa r a t i v e Immun o l o g y 15:
  2. (1997). and Trencz e k , T.,
  3. (1999). Apolip o p h o r i n - I I I in Galleria mellonella potent i a t e s hemol y mp h lytic activ i t y . Devel o p me n t a l & Compa r a t i v e Immun o l o g y .
  4. (2000). Apolipo p h o r i n - I I I and the inter a c t i o n s of lipot e i c h o i c acids with the immed i a t e immun e respo n s e s of Galleria mellonella. Journ a l of Inver t e b r a t e Patho l o g y .
  5. (2000). Apolipophorin-III and the interactions of lipoteichoic acids with the immediate immune responses of Galleria mellonella. doi
  6. (1997). Biological mediators of insect immunity. doi
  7. (2001). D e t t l o f f
  8. (1988). Effects Invi vo of Beta-1 , 3 - G l u c a n s from Fungal CellWa l l s on the Circu l a t i n g Hemoc y t e s of the Deser t Locus t Schis t o c e r c a - Grega r i a . ”
  9. (1988). Effects Invivo of Beta-1,3-Glucans from Fungal CellWalls on the Circulating Hemocytes of the Desert Locust Schistocerca - doi
  10. (1983). Goldsw o r t h y
  11. (1985). Gunnar s s o n
  12. (1997). Haemo l y mp h protei n s of larvae of Galler i a mello n e l l a detox i f y endot o x i n s of the insec t patho g e n i c bacte r i a Xenor h a b d u s nemat o p h i l u s (Ente r o b a c t e r i a c e a e ) .
  13. (1997). Haemolymph proteins of larvae of Galleria mellonella detoxify endotoxins of the insect pathogenic bacteria Xenorhabdus nematophilus doi
  14. (1985). Hemocytic Aggregation in Schistocerca-Gregaria and Periplaneta- Americana as a Response to Injected Substances of Microbial Origin.” doi
  15. (1980). Induct i o n of antimi c r o b i a l activi t y in the blood of the migrat o r y locus t Locusta migratoria L. Journa l of Insect Physio l o g y 26: 539-5
  16. (1980). Induction of antimicrobial activity in the blood of the migratory locust Locusta migratoria L. doi
  17. (1989). Insec t Hemoly mp h -Coope r a t i o n betwe e n Humor a l and Cellu l a r Facto r s in Locus t a - M i g r a t o r i a .
  18. (1996). Insect Hemocyte Adhesion In Vitro: Inhibition by Apoliphorin I and an doi
  19. (1989). Insect Hemolymph -Cooperation between Humoral and Cellular Factors in doi
  20. (1997). Isolate d apoli p o p h o r i n III from Galleria mellonella stimul a t e s the immun e react i o n s of this insec t . Journ a l of Insec t Physi o l o g y 43: 383-3
  21. (1997). Isolated apolipophorin III from Galleria mellonella stimulates the immune reactions of this insect. doi
  22. (1994). Kadonoo k u d a , K., Hirama t s
  23. (1996). Linea r i z a t i o n of the Bradfo r d prote i n assay incre a s e s its sensi t i v i t y : Theor e t i c a l and exper i me n t a l studi e s . Analy t i c a l Bioch e mi s t r y 236:
  24. (1996). Linearization of the Bradford protein assay increases its sensitivity: Theoretical and experimental studies. doi
  25. (1992). Lipoph o r i n inhibi t s the adhesi o n of cockro a c h Perip l a n e t a - ameri c a n a hemoc y t e s in vitro . Journ a l of Insec t Physi o l o g y
  26. (1992). Lipophorin inhibits the adhesion of cockroach Periplaneta- americana hemocytes in vitro. doi
  27. (2001). Localization of injected Apolipophorin III in vivo - new insights into the immune activation process directed by this protein. doi
  28. (1996). o n e s doi
  29. (2001). Physiology and endocrine control of flight. In: Insect movement: mechanisms and consequences, doi
  30. (1999). Plasma lipop r o t e i n s promo t e the relea s e of bacte r i a l lipop o l y s a c c h a r i d e from the monoc y t e cell surfa c e . Journ a l of Biolo g i c a l Chemi s t r y 274: 34116-
  31. (1999). Plasma lipoproteins promote the release of bacterial lipopolysaccharide from the monocyte cell surface. doi
  32. (1994). Shimabu k u r o
  33. (1997). Struc t u r e s , assay s and recep t o r s for locus t adipo k i n e t i c hormo n e s . Compa r a t i v e Bioch e mi s t r y and Physio l o g y - B Bioche mi s t r y and Molecu l a r Biolo g y 117:
  34. (1997). Structures, assays and receptors for locust adipokinetic hormones. Comparative Biochemistry and Physiology - doi
  35. (1983). The endocrine control of flight metabolism in locusts. doi
  36. (1998). Two major prote i n s from locus t plasma are invol v e d in coagu l a t i o n and are speci f i c a l l y preci p i t a t e d by lami n a r i n , a beta- 1 , 3 -g l u c a n .
  37. (1998). Two major proteins from locust plasma are involved in coagulation and are specifically precipitated by laminarin, a beta-1,3-glucan.” Insect Biochemistry and Molecular doi

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