Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays


A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection

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Repositorio Institucional de la Universidad de Oviedo

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oaioai:digibuo.uniovi.es:10651/10668Last time updated on 11/29/2016

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