Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

Abstract

We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns

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Last time updated on 11/21/2016View original full text link

This paper was published in UNT Digital Library.

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