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In vitro anti-melanogenesis effect of crude extract from seed coat of black soybean toward human malignant melanoma A375.S2 cell line

By 詹鈞凱

Abstract

[[abstract]]本論文主要探討黑豆(台南三號)之種皮50%乙醇萃取物對酪胺酸酵素活性抑制影響,並利用人類黑色素瘤細胞A375.S2細胞株,進一步研究黑豆種皮50%乙醇萃取物對體外黑色素生成之抑制影響。於酪胺酸酵素活性抑制實驗之結果顯示,黑豆50%乙醇萃取物對酪胺酸酵素活性有抑制影響,於同濃度(1.66 mg/ml)下黑豆種皮50%乙醇萃取物對於酪胺酸酵素活性抑制率為黑豆整顆50%乙醇萃取物之兩倍以上,顯示大部份之抑制酪胺酸酵素有效成份存在於黑豆種皮中,且抑制率隨著黑豆種皮50%乙醇萃取物濃度提高而增加,抑制機制屬於混合型抑制作用。於A375.S2細胞培養試驗之結果顯示,A375.S2細胞株中含有酪胺酸酵素活性可與受質進行反應生成黑色素前驅物,於黑豆50%乙醇萃取物加藥處理後,以三種不同濃度之L-3,4-dihydroxy- phenylalanine (L-dopa) 0.05, 0.25 and 0.5 mg/ml作為受質,最高的兩個黑豆50%乙醇萃取物濃度(20 μg/ml與30 μg/ml)對於A375.S2細胞株中酪胺酸酵素活性有較明顯的抑制效果

Publisher: 亞洲大學
Year: 2009
OAI identifier: oai:localhost:310904400/2004
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