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Aptamers and SELEX: the technology

By Tim Sampson

Abstract

DNA and RNA microarrays have become an important analytical technique in the understanding and characterisation of genomes and transcriptomes. A recent development in this field of combinatorial chemistry has concentrated on using artificial DNA/RNA sequences--aptamers--as the screening ligand. In reviewing this technology, the article covers the topic, following a general introduction, under the headings: generating an aptamer library, aptamer library complexity, aspects of nucleotide chemistry, constant region primer design, and the SELEX protocol for exposing an aptamer library to the desired target.Aptamers SELEX protocol Spiegelmers Combinatorial chemistry Oligonucleotide ligands Screening library Molecular evolution Exponential enrichment Microarrays

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