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Effect of photoperiod and host distribution on the horizontal transmission of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in greenhouse whitefly assessed using a novel model bioassay

By P.B. Avery, G.L. Queeley, Jane Nicklin and M.S.J. Simmonds


A model bioassay was used to evaluate the epizootic potential and determine the horizontal transmission efficiency of Isaria fumosorosea Trinidadian strains against Trialeurodes vaporariorum pharate adults under optimum conditions (25±0.5°C, ~100% RH) at two different photoperiods. Untreated pharate adults were arranged on laminated graph paper at different distributions to simulate varying infestation levels on a leaf surface. Four potential hosts were located 7, 14 and 21 mm away from a central sporulating cadaver simulating high, medium and low infestation levels, respectively. Percent hosts colonized were recorded 7, 12, 14 and 21 days post-treatment during a 16- and 24-h photophase. After 21 days, mean percent hosts colonized at the highest, middle and lowest infestation levels were 93 and 100%, 22 and 58%, 25 and 39% under a 16- and 24-h photophase, respectively. From the results, it was concluded that the longer the photophase, the greater the percentage of hosts colonized, and as host distance increased from the central sporulating cadaver, colonization decreased. The use of this novel model bioassay technique is the first attempt to evaluate the epizootic potential and determine the horizontal transmission efficiency of I. fumosorosea Trinidadian strains under optimal environmental conditions at different photoperiods. This bioassay can be used to assess horizontal transmission efficiency for the selection of fungi being considered for commercial biopesticide development

Topics: bcs
Publisher: Taylor & Francis
Year: 2010
OAI identifier: oai:eprints.bbk.ac.uk.oai2:1258

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  7. (1995). Conidial discharge and transmission efficiency of Neozygites floridana, an entomopathogenic fungus infecting two-spotted spider mites under laboratory conditions. doi
  8. (1997). Control of white grubs (Melolontha melolontha L.) by treating adults with the fungus Beauveria brongniartii. doi
  9. (1999). Dependence of the entomopathogenic fungus, Beauveria bassiana, on high humidity for infection of Rhodnius prolixus. doi
  10. (1984). Detection of a natural fungal epizootic in the whitefly, Trialeurodes vaporariorum Westwood.
  11. (2008). Ecological approaches to pest management using entomopathogenic fungi; concepts, theory, practice and opportunities.
  12. (2003). Effect of air humidity on the infection potential of hyphomycetous fungi as mycoinsecticides for Trialeurodes vaporariorum. doi
  13. (2004). Effect of different photoperiods on the growth, infectivity and colonization of Trinidadian fungal strains and spore types of Paecilomyces fumosoroseus on the greenhouse whitefly, Trialeurodes vaporariorum, using a glass slide bioassay. doi
  14. (2001). Effect of dose, pre-mortem host incubation temperature and thermal behaviour on host mortality, mycosis and sporulation of Metarhizium anisopliae var. acridum in Schistocerca gregaria. doi
  15. (2000). Effect of host plant on Beauveria bassiana- and Paecilomyces fumosoroseus-induced mortality of Trialeurodes vaporariorum (Homoptera: Aleyrodidadae). doi
  16. (1985). Effect of light on the conidiation of Paecilomyces fumosoroseus.
  17. (2001). Effects of temperature and relative humidity on sporulation of Metarhizium anisopliae var. acridum in mycosed cadavers of Schistocerca gregaria. doi
  18. (2003). Entomopathogenic fungi as biocontrol agents. doi
  19. (1984). Epizootic potential for aphids of different isolates of the fungus, Verticillium lecanii. doi
  20. (2006). Epizootics of an entomopathogenic fungus Entomophthora aphidis Hoggman on the pomegranate aphid Aphis punicae passerine (Homoptera: Aphididae.
  21. (2000). Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemisia argentifolii. doi
  22. (1991). First report in Mexico on the isolation of Verticillium lecanii from whitefly and in vitro pathogenicity tests on this insect.
  23. (1987). Fungal diseases.
  24. (2008). Further compatibility tests of the entomopathogenic fungus Lecanicillium muscarium with conventional insecticide products for control of sweet potato whitefly, Bemisia tabaci on poinsettia plants. doi
  25. (1999). Germination of conidia and blastospores of Paecilomyces fumosoroseus on the cuticle of the silverleaf whitefly, Bemisia argentifolii.
  26. (1995). Global distribution of naturally occurring fungi of Bemisia, their biologies and use as biological control agents.
  27. (2000). Horizontal transmission of Beauveria bassiana (Bals.) Vuill. Agricultural and Forest Entomology doi
  28. (2004). Horizontal transmission of the entomopathogenic fungus Beauveria bassiana among the spruce bark beetle, Ips typographus (Col., Scolytidae) in the laboratory and under field conditions. doi
  29. (2000). Host plant effects on activity of the mitosporic fungi Beauveria bassiana and Paecilomyces fumosoroseus against two populations of Bemisia whiteflies (Homoptera: Aleyrodidae).
  30. (1997). In vitro effects of secondary plant compounds on germination of blastospores of the entomopathogenic fungus Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes). doi
  31. (2004). Influence of humidity on efficacy of entomopathogenic fungi-case study “Paecilomyces fumosoroseus-Trialeurodes vaporariorum”.
  32. (1992). Insecticide resistance in field populations of greenhouse whitefly (Homoptera: Aleyrodidae) in the San Joaquin Valley, California cotton cropping system. doi
  33. (1993). Integrated pest management in glasshouse ornamentals in the Netherlands: a step by step policy.
  34. (2000). Laboratory evaluation of the impact of entomopathogenic fungi on Prorops nasuta (Hymenoptera: Bethylidae), a parasitoid of the coffee berry borer. doi
  35. (1997). Liquid culture production of desiccation tolerant blastospores of the bioinsecticidal fungus Paecilomyces fumosoroseus. doi
  36. (2000). Modeling Beauveria bassiana horizontal transmission. Agriculture and Forest Entomology 2: doi
  37. (1997). Modelling fungal (Neozygites cf. floridana ) epizootics in local populations of cassava green mites (Mononychellus tanajoa ). doi
  38. (1992). Monitoring insecticide resistance in greenhouse whitefly (Homoptera: Aleyrodidae) with yellow sticky cards.
  39. (2002). Pathogenicity of Aschersonia spp. against whiteflies Bemisia argentifolii and Trialeurodes vaporariorum. doi
  40. (2005). Pathogenicity of Paecilomyces spp. to the glasshouse whitefly, Trialeurodes vaporariorum, with some observations on the fungal infection process.
  41. (1998). Pathogenicity of the entomopathogenic fungi Paecilomyces spp. and Beauveria bassiana against the silverleaf whitefly, Bemisia argentifolii. doi
  42. (2005). Pathogenicity of the entomopathogenic fungus, Lecanicillium muscarium, against the sweet potato whitefly Bemisia tabaci under laboratory and glasshouse conditions. doi
  43. (1992). Pelargonium cultivar selection by adults of greenhouse whitefly (Homoptera: Aleyrodidae). doi
  44. (2000). Persistence of the fungal whitefly pathogen, Aschersonia aleyrodis, on three different plant species. doi
  45. (1995). Pirimiphos-methyl residues and control of greenhouse whitefly (Homoptera: Aleyrodidae) on seven vegetables.
  46. (2005). Preliminary experiments to control the silverleaf whitefly with electrostatic spraying of a mycoinsecticide. doi
  47. (1996). Production of Paecilomyces fumosoroseus conidia in submerged culture. doi
  48. (1999). Sporulation of Beauveria bassiana on cadavers of Triatoma infestans after infection at different temperatures and doses of inoculum. doi
  49. (1999). Supplemental lighting of greenhouse vegetables: limitations and problems related to long photoperiods.
  50. (2003). Susceptibility of immature and adult stages of Trialeurodes vaporariorum (Hom., Aleyrodidae) to selected insecticides. doi
  51. (1997). Temperature and moisture requirements for conidial germination of an isolate of Beauveria bassiana, pathogenic to Rhodnius prolixus. doi
  52. (1998). The effect of selected allelochemicals on germination of conidia and blastospores and mycelial growth of the entomopathogenic fungus, Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes).
  53. (2002). Tritrophic interactions among Paecilomyces fumosoroseus, Encarsia formosa and Trialeurodes vaporariorum on Phaseolus vulgaris and Pelargonium spp.

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