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A new crystal form of penicillin acylase from Escherichia coli

By C.E. McVey, S.P. Tolley, S.H. Done, J.A. Brannigan and K.S. Wilson


A new crystal form of penicillin acylase (penicillin amidohydrolase, E.C. from Escherichia coli W (ATCC 11105) is reported. The crystals were grown using a combination of hanging-drop and streak-seeding methods. The crystals are in the monoclinic space group P2(1) with cell dimensions a = 51.52, b = 131.95, c = 64.43 Angstrom, beta = 106.12 degrees. There is one heterodimer in the asymmetric unit (Vm = 2.45 Angstrom(3) Da(-1)) and the solvent content is 49%. Preliminary data have been collected to d(min) = 2.7 Angstrom using a MAR Research image plate and a rotating-anode X-ray source. Subsequent experiments show diffraction beyond 1.3 Angstrom at a synchrotron radiation source

Year: 1997
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