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Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A

By K Muchova, R J Lewis, J A Brannigan, W A Offen, D P Brown, I Barak, P Youngman and A J Wilkinson


The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein and subsequently crystallized in forms suitable for X-ray crystallographic studies. The DNA-binding domain has been crystallized in two forms, one of which diffracts X-rays to beyond 2.5 Angstrom spacing. The crystals of the phospho-acceptor domain diffract X-rays beyond 2.0 Angstrom spacing using synchrotron radiation

Year: 1999
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