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The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken

By K. Bruce, F.A. Myers, E. Mantouvalou, P. Lefevre, I. Greaves, C. Bonifer, D.J. Tremethick, A.W. Thorne and C. Crane-Robinson

Abstract

The replacement histone H2A.Z is variously reported\ud as being linked to gene expression and preventing the\ud spread of heterochromatin in yeast, or concentrated\ud at heterochromatin in mammals. To resolve this\ud apparent dichotomy, affinity-purified antibodies\ud against the N-terminal region of H2A.Z, in both a triacetylatedandnon-\ud acetylatedstate, areusedin native\ud chromatin immmuno-precipitation experiments with\ud mononucleosomes from three chicken cell types. The\ud hyperacetylated species concentrates at the 50 end of\ud active genes, both tissue specific and housekeeping\ud but is absent from inactive genes, while the\ud unacetylated form is absent from both active and\ud inactive genes. A concentration of H2A.Z is also\ud found at insulators under circumstances implying a\ud link to barrier activity but not to enhancer blocking.\ud Although acetylated H2A.Z is widespread throughout\ud the interphase genome, at mitosis its acetylation is\ud erased, the unmodified form remaining. Thus,\ud although H2A.Z may operate as an epigenetic marker\ud for active genes, its N-terminal acetylation does not

Year: 2005
OAI identifier: oai:eprints.whiterose.ac.uk:968

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