Skip to main content
Article thumbnail
Location of Repository

Flow injection determination of readily assimilable nitrogen compounds during vinification

By Jose Gonzalez-Rodriguez, Maria Dolores Luque de Castro and Pedro Perez Juan


A flow injection method for the determination of readily assimilable nitrogen (r.a.n.), i.e. ammonium and aminated nitrogen, is reported. The difference in pH of the sample in the presence and absence of formaldehyde, which blocks the amino function, provides the value of r.a.n. by monitoring the changes in absorbance of bromothymol blue at 616 nm. The detection and quantification limits are 10 and 11.6 mg l-1, respectively; the reproducibility and repeatability are 3.94 mg l-1 and 1.35 mg l-1, respectively; and the sample throughput is 20 samples h-1. The method has been applied to the analysis of 120 samples of must and wine subjected to biological aging. The proposed method also provides good correlation with the reference method used in routine analysis, and it is faster and gives sufficient precision for wineries requirements

Topics: F110 Applied Chemistry, F111 Industrial Chemistry, F190 Chemistry not elsewhere classified, F100 Chemistry, F180 Analytical Chemistry
Publisher: Royal Society of Chemistry, Thomas Graham House, Science Park, Milton Rd, Cambridge CB4 0WF
Year: 2002
DOI identifier: 10.1039/b110686b
OAI identifier:

Suggested articles


  1. (2000). Enología: Fundamentos científicos y tecnológicos, AMV Ed.
  2. (2000). Handbook of enology. Volume 2. The Chemistry of wine. Stabilization and treatments, doi
  3. (1990). Recueil des méthodes Internationales d´Analyse des Vins et des Mouts, Office International de la vigne et du vin (OIV),
  4. (1987). Trattato di Scienzia e Tecnica Enologica, Vol I, Analisi e controllo dei mosti e dei vini, AEB

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.