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Flow injection determination of readily assimilable nitrogen compounds during vinification

By Jose Gonzalez-Rodriguez, Maria Dolores Luque de Castro and Pedro Perez Juan

Abstract

A flow injection method for the determination of readily assimilable nitrogen (r.a.n.), i.e. ammonium and aminated nitrogen, is reported. The difference in pH of the sample in the presence and absence of formaldehyde, which blocks the amino function, provides the value of r.a.n. by monitoring the changes in absorbance of bromothymol blue at 616 nm. The detection and quantification limits are 10 and 11.6 mg l-1, respectively; the reproducibility and repeatability are 3.94 mg l-1 and 1.35 mg l-1, respectively; and the sample throughput is 20 samples h-1. The method has been applied to the analysis of 120 samples of must and wine subjected to biological aging. The proposed method also provides good correlation with the reference method used in routine analysis, and it is faster and gives sufficient precision for wineries requirements

Topics: F110 Applied Chemistry, F111 Industrial Chemistry, F190 Chemistry not elsewhere classified, F100 Chemistry, F180 Analytical Chemistry
Publisher: Royal Society of Chemistry, Thomas Graham House, Science Park, Milton Rd, Cambridge CB4 0WF
Year: 2002
DOI identifier: 10.1039/b110686b
OAI identifier: oai:eprints.lincoln.ac.uk:2116

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Citations

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