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An efficient and versatile solid phase synthesis of 5'- and 3'-conjugated oligonucleotides

By J. D'ONOFRIO, D. MONTESARCHIO, L. DE NAPOLI and G. DI FABIO

Abstract

An easy and efficient solid-phase strategy to obtain 5¢- and 3¢-oligonucleotide conjugates in highly pure form has been developed. Ad hoc derivatized solid supports, to which the first nucleoside unit can be attached through a phosphate linkage, have been exploited both in a preand post-DNA assembly conjugation approach. A number of 5'- or 3'- oligonucleotide conjugates, incorporating a variety of labels covalently linked through a phosphodiester or a phosphoramidate bond, have been synthesized and characterized

Topics: solid-phase synthesis, oligonucleotide conjugates, phosphoramidate
Publisher: 'American Chemical Society (ACS)'
Year: 2005
DOI identifier: 10.1021/ol051811g
OAI identifier: oai:www.iris.unina.it:11588/204736
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