Preliminary xylitol separation tests were carried out using both xylitol-xylose synthetic solutions and fermented hardwood hemicellulose hydrolyzate. Xylitol was recovered by a crystallization methodology consisting of dilute solutions evaporation up to supersaturation, supersaturated solutions cooling, separation of crystals by centrifugation, and final filtration. Besides, xylitol solubility limits were estimated at different crystallization temperatures. Product yields and crystal purity were calculated and crystallization kinetics were investigated
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