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PEPTIDE-15 STIMULATES PULP STEM CELLS TOWARDS OSTEOBLASTS DIFFERENTIATION

By Vincenzo Sollazzo, Stefano Fanali, Elena Masiero, Ambra Girardi, Francesca Farinella, Elisabetta Melloni, Furio Pezzetti, Corrado Iaccarino, Giorgio Zauli and Francesco Carinci

Abstract

Peptide-15 (P-15) is a bone augmentation material analog to the cell binding domain of collagen. P-15 competes for cell surface sites for attachment of collagen and, when immobilized on surfaces, it promotes adhesion of cells and facilitates physiological process like binding, migration and differentiation of cells. To study how P15 can induce osteoblast differentiation and proliferation in mesenchimal stem cells, the expression levels of bone related genes ( RUNX2,SP7,ALPL,SPP1,COL1A1,COL3A1 and FOSL1)and mesenchimal stem cells marker (ENG) were measured in Dental Pulp Stem Cells (DPSCs) and Normal Osteoblast (NO),after 15and 30 days of treatment. Significantly, differentially expressed genes among DPSCs and NO were SP7,ENG,RUNX2,COL3A1,COL1A1,ALPL,and SPP1 in the first 15 days of treatment and ENG,RUNX2,COL3A1,SPP1 and ALPLafter 30 days. Theresent study demonstrated that P15 influences the behaviour of DPSCs in vitro by enhancing proliferation, differentiation and deposition of matri

Topics: P15, stem cells, biomaterial, gene expression, differentiation
Year: 2011
OAI identifier: oai:iris.unife.it:11392/1506328
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