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TRICALCIUM PHOSPHATE STIMULATES ADIPOSE TISSUE-DERIVED STEM CELLS TOWARDS OSTEOBLASTS DIFFERENTIATION

By V. SOLLAZZO, S. FANALI, E. MASIERO, A. GIRARDI, F. FARINELLA, E. MELLONI, F. PEZZETTI, C. IACCARINO, G. ZAULI and F. CARINCI

Abstract

Tricalcium Phosphate (TCP) is used successfully as bone substitutes and scaffolds for tissue engineering and implantology to its capacity to enhance bone formation in vivo. To study how TCP can induce osteoblast differentiation and proliferation in mesenchymal stem cells, the expression levels of bone related genes were mesasured in adipose derived stem cells (ADSCs) and normal osteoblast(NO) cultivated on TCP scaffolds after 15 and 30 days of treatment using real time Reverse Transcription-Polymerase Chain Reaction. Significantly, differentially expressed genes among ADSCs and NO were RUNX,COL1A1,ALPL and SPP1 in the first 15 days of tratment and SP7,FOSL1,RUNX2,COL3A1,COL1A1,SPP1 and ALPL after 30 days. The present study demonstrated that TCP influences the behavior ofADSCs in vitro by enhancing proliferation, differentiation and deposition of matri

Topics: Tricalcium Phosphate, adipose tissue, stem cells, gene expression, PCR real time
Year: 2011
OAI identifier: oai:iris.unife.it:11392/1506113
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