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A simple method to identify bone marrow mesenchymal stromal cells with a high immunosuppressive potential.

By Rizzo R., Lanzoni G., Stignani M., Campioni D., Alviano F., Ricci F., Tazzari P.L., Melchiorri L., Zaccaria Scalinci S., Cuneo A., Bonsi L., Lanza F., Bagnara G.P. and Baricordi O.R.


Background: The beneficial activity of mesenchymal stromal cells (MSC) in allogeneic hematopietic stem cell transplantation requires a correct use in terms of cell dose and timing of infusion and the identification of biomarkers for selection. The immunosuppressive bone marrow derived BM-MSC functions has been associated with the production of soluble HLA-G molecule (sHLA-G), via interleukin-10 (IL-10). We established a reliable method to evaluate BM-MSC HLA-G expression without the influence of peripheral blood mononuclear cells (PBMCs). Methods: Thirteen BM-MSCs from donors were activated with recombinant IL-10 or co-cultured with 10 different phytohaemaglutinin treated-peripheral blood mononuclear cells (PHA-PBMCs). Membrane-bound and soluble HLA-G expression was evaluated by flow cytometry and ELISA respectively; lymphoproliferation was measured by (methyl-3H) Thymidine. \ud Results: The results demonstrated the ability of IL-10 to stimulate both membrane-bound and soluble HLA-G production by BM-MSCs. The levels of HLA-G expression induced by IL-10 in BM-MSCs were associated with the inhibition of PHA-PBMC proliferation (sHLA-G: p= 0.0008, r=0.9308; membrane HLA-G: p= 0.0005, r= 0.9502). \ud Discussion: These results propose the evaluation of sHLA-G production in IL-10-treated BM-MSC cultures as a possible marker of immunoregulatory functions

Year: 2011
DOI identifier: 10.3109/14653249.2010.542460
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