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Polysaccharide determination in protein/polysaccharide mixtures for phase-diagram construction

By Jacob K. Agbenorhevi and Vassilis Kontogiorgos

Abstract

The effect of protein removal from protein–polysaccharide mixtures on the determination of total\ud sugar concentration for phase-diagram construction was explored by response surface methodology.\ud Phenol–sulphuric acid method was used to estimate the total sugar concentration whereas phase separation\ud and microstructure of biopolymers in the mixtures were probed by means of fluorescent microscopy.\ud All the factors studied (trifluoroacetic acid (TFA) volume, protein concentration, precipitation time) had\ud significant influence on the polysaccharide recovery from the mixtures. The overall pattern of protein\ud precipitation with TFA was also found to highly depend on protein–time interactions. Images taken at different\ud mixture concentrations and with two different filter sets revealed a phase-separated system with\ud the guar gum domains surrounded by a continuous protein phase. Experimental measurements as well as\ud microscopical evidence suggests that, for greater than 60% polysaccharide recovery (±6% error), the protein\ud content in the mixtures should be <∼5.5% (w/v) with less than 0.5mL TFA combined with more than\ud 30 minrest under the influence of TFA. The results of the present investigationshowedthatwhenattempting\ud to construct phase diagram of mixed biopolymer systems the equilibrium phases must be diluted to\ud a protein content <∼5% (w/v) before TFA treatment if the maximum polysaccharide concentration is to\ud be determined

Topics: QD
Publisher: Elsevier
Year: 2010
OAI identifier: oai:eprints.hud.ac.uk:8967

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