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Apoptosis in mouse fetal and neonatal oocytes during meiotic \ud prophase one

By Fataneh Ghafari, Carlos G. Gutierrez and Geraldine M. Hartshorne

Abstract

Background \ud The vast majority of oocytes formed in the fetal ovary do not survive beyond birth. \ud Possible reasons for their loss include the elimination of non-viable genetic \ud constitutions arising through meiosis, however, the precise relationship between \ud meiotic stages and prenatal apoptosis of oocytes remains elusive. We studied oocytes \ud in mouse fetal and neonatal ovaries, 14.5–21 days post coitum, to examine the \ud relationship between oocyte development and programmed cell death during meiotic \ud prophase I. \ud Results \ud Microspreads of fetal and neonatal ovarian cells underwent immunocytochemistry for \ud meiosis- and apoptosis-related markers. COR-1 (meiosis-specific) highlighted axial \ud elements of the synaptonemal complex and allowed definitive identification of the \ud stages of meiotic prophase I. Labelling for cleaved poly-(ADP-ribose) polymerase \ud (PARP-1), an inactivated DNA repair protein, indicated apoptosis. The same oocytes \ud were then labelled for DNA double strand breaks (DSBs) using TUNEL. 1960 \ud oocytes produced analysable results. . \ud Oocytes at all stages of meiotic prophase I stained for cleaved PARP-1 and/or TUNEL, or neither. Oocytes with fragmented (19.8%) or compressed (21.2%) axial \ud elements showed slight but significant differences in staining for cleaved PARP-1 and \ud TUNEL to those with intact elements. However, fragmentation of axial elements \ud alone was not a good indicator of cell demise. Cleaved PARP-1 and TUNEL staining \ud were not necessarily coincident, showing that TUNEL is not a reliable marker of apoptosis in oocytes. \ud \ud Conclusions \ud Our data indicate that apoptosis can occur throughout meiotic prophase I in mouse \ud fetal and early postnatal oocytes, with greatest incidence at the diplotene stage. \ud Careful selection of appropriate markers for oocyte apoptosis is essential

Topics: QH301, QH426
Publisher: Biomed Central Publisher
Year: 2007
OAI identifier: oai:wrap.warwick.ac.uk:104

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