Location of Repository

Improving sensitivity of oral fluid testing in IgG prevalence studies: application of mixture models to a rubella antibody survey \ud

By N. J. Gay, A. J. Vyse, F. Enquselassie, Wondatir Nigatu and D. James Nokes


A method for the analysis of age-stratified antibody prevalence surveys is applied to a previously reported survey of antibody to rubella virus using oral fluid samples in which the sensitivity of the assay used was shown to be compromised. The age-specific distribution of the quantitative results of antibody tests using oral fluids is modelled as a mixture of strong positive, weak positive and negative components. This yields maximum likelihood estimates of the prevalence at each age and demonstrates that, when used in conjunction with mixture modelling techniques, the results of antibody prevalence studies using oral fluids accurately reflect those obtained using sera

Topics: QR355
Publisher: Cambridge University Press
Year: 2003
OAI identifier: oai:wrap.warwick.ac.uk:777

Suggested articles



  1. (1997). A comparison of oral fluid and serum for the detection of rubellaspecific antibodies in a community study in Addis Ababa, Ethiopia. Trop Med Int Health doi
  2. (1996). A school outbreak of parvovirus B19 infection investigated using salivary antibody assays. Epidemiol Infect doi
  3. (1998). An evaluation of oral-fluid collection devices forthedeterminationofrubellaantibodystatusinarural Ethiopian community. Trans Roy Soc Trop Med Hyg doi
  4. (1996). Analysis of serological surveys using mixture models: application to a survey of parvovirus B19. Stat Med doi
  5. (1997). Detection of IgG antibody to Epstein–Barr virus viral capsid antigen insalivabyantibodycaptureradioimmunoassay.JVirol Meth doi
  6. (1999). Detection of measles specific IgG in oral fluid using an FITC/ anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA). doi
  7. (1993). Detection of measles, mumps and rubella doi
  8. (1999). Detection of rubella virus-specific immunoglobulin G in saliva by an amplification-based enzyme-linked immunosorbent assay using monoclonal antibody to fluorescein isothiocyanate.
  9. (1989). Diagnosis of hepatitis A and B by testing saliva. doi
  10. (1991). MortimerP,ParryJ.Non-invasivevirologicaldiagnosis: are saliva and urine specimens adequate substitutes for blood? Rev Med Virol
  11. (2001). NokesDJ,EnquselassieF,NigatuW,etal.Hasoralfluid the potential to replace serum for the evaluation of population immunity levels? A study of measles, rubella and hepatitis B in rural Ethiopia. Bull WHO
  12. Oral diagnostic testing for detecting human immunodeficiency virus-1 antibodies: a technologywhosetimehascome.AmJMed1997;102:9–14. doi
  13. (1988). Surveillance of antibody to measles, mumps and rubella by age. doi
  14. (1996). WangJ,Adler S.Salivary antibodiestocytomegalovirus (CMV) glycoprotein B accurately predict CMV infections among preschool children.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.