Location of Repository

Molecular studies on intraspecific diversity and phylogenetic position of Coniothyrium minitans

By S. Muthumeenakshi, Alan L. Goldstein, Alison Stewart and J. M. Whipps

Abstract

Simple sequence repeat (SSR)±PCR amplification using a microsatellite primer (GACA)% and ribosomal RNA gene sequencing were used to examine the intraspecific diversity in the mycoparasite Coniothyrium minitans based on 48 strains, representing eight colony types, from 17 countries world-wide. Coniothyrium cerealis, C. fuckelii and C. sporulosum were used for interspecific comparison. The SSR±PCR technique revealed a relatively low level of polymorphism within C. minitans but did allow some differentiation between strains. While there was no relationship between SSR±PCR profiles and colony type, there was some limited correlation between these profiles and country of origin. Sequences of the ITS 1 and ITS 2 regions and the 5±8S gene of rRNA genes were identical in all twenty-four strains of C. minitans examined irrespective of colony type and origin. These results indicate that C. minitans is genetically not very variable despite phenotypic differences. ITS and 5±8S rRNA gene sequence analyses showed that C. minitans had similarities of 94% with C. fuckelii and C. sporulosum (which were identical to each other) and only 64% with C. cerealis. Database searches failed to show any similarity with the ITS 1 sequence for C. minitans although the 5±8S rRNA gene and ITS 2 sequences revealed an 87% similarity with Aporospora terricola. The ITS sequence including the 5±8S rRNA gene sequence of Coniothyrium cerealis showed 91% similarity to Phaeosphaeria microscopica. Phylogenetic analyses using database information suggest that C. minitans, C. sporulosum, C. fuckelii and A. terricola cluster in one clade, grouping with Helminthosporium species and 'Leptosphaeria' bicolor. Coniothyrium cerealis grouped with Ampelomyces quisqualis and formed a major cluster with members of the Phaeosphaeriacae and Phaeosphaeria microscopica

Topics: QR
Publisher: Cambridge University Press
Year: 2001
OAI identifier: oai:wrap.warwick.ac.uk:821

Suggested articles

Preview

Citations

  1. (1947). A new species of Coniothyrium parasitic on sclerotia. doi
  2. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies on nucleotide sequences. doi
  3. (1999). A simpli®ed material and energy balance approach to process development and scale-up of Coniothyrium minitans conidia production by solid-state cultivation in packed-bed reactor. doi
  4. (1990). Ampli®cation and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A guide to methods and applications doi
  5. (1997). Biological control of Sclerotinia sclerotiorum by ®lm-coating Coniothyrium minitans on seed and sclerotia. Plant Pathology 46: 919±929.Intraspeci®c diversity doi
  6. (1992). Biology of Coniothyrium minitans and its potential for use in disease biocontrol. doi
  7. (1996). Characterization of Colletotrichum gloeosporioides isolates from avocado and almond fruits with molecular and pathogenicitytests.AppliedandEnvironmentalMicrobiology62:1014±1020.
  8. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-speci®c gap penalties and weight matrix choice. doi
  9. (1999). Co-transformation of the sclerotial mycoparasite Coniothyrium minitans with hygromycin B resistance and B-glucuronidase markers. doi
  10. (1994). Comparative analysis of molecular and biological characteristics of strains of Beauveria brongniartii isolated from insects. doi
  11. (1995). Comparison of nuclear ribosomal DNA sequences from Alternaria species pathogenic to crucifers. doi
  12. (1980). Compendium of Soil Fungi. doi
  13. (1985). Con®dence limits on phylogenies: an approach using the bootstrap. doi
  14. (1974). Concerning some species of Microsphaeropsis. doi
  15. (1982). Coniothyrium minitans.
  16. (1998). Cryptic species in the Puccinia monoica complex. doi
  17. (1999). Delineation of Trichoderma harzianum into two different genotypic groups by a highly robust ®ngerprinting method, UP±PCR, and UP±PCR product cross-hybridization. doi
  18. (1999). Determination of Coniothyrium minitans conidial and germling lectin affinity by ¯ow cytometry and digital microscopy. doi
  19. (1999). DNA polymorphism and host range in the take-all fungus, Gaeumannomyces graminis. doi
  20. (1993). DNA- and PCR-®ngerprinting in fungi. doi
  21. (2000). Effect of the availability of magnesium ions in K-carrageenan gels on the formation of conidia by Coniothyrium minitans. doi
  22. (1997). Effects of culture media and environmental factors on conidial germination, pycnidial production and hyphal extension of Coniothyrium minitans. doi
  23. (1993). Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis. doi
  24. (1998). Genetic comparison of the aggressive weed mould strains of Trichoderma harzianum from mushroom compost in North America and the British Isles. doi
  25. (1996). Genetic homogeneity of cultivated strains of shiitake (Lentinula edodes) used in China as revealed by the polymerase chain reaction. doi
  26. (1994). Genotypic characterization of sequential Candida albicans isolates from ¯uconazole-treated neutropenic patients. doi
  27. (1991). Glasshouse trials of Coniothyrium minitans and Trichoderma species for the biological control of Sclerotinia sclerotiorum in celery and lettuce. doi
  28. (1998). Hydrophobicity and surface charge of conidia of the mycoparasite Coniothyrium minitans. doi
  29. (1998). Identi®cation and phylogeny of spore-cyst fungi (Ascosphaera spp.) using ribosomal DNA sequences. doi
  30. (1994). Independent origins of the synnematous Penicillium species, doi
  31. (1994). Intraspeci®c molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles. doi
  32. (1999). Long-term biosanitation by application of Coniothyrium minitans on Sclerotinia sclerotiorum-infected crops. doi
  33. (1999). Medium optimization for spore production of Coniothyrium minitans using statistically-based experimental designs. doi
  34. (1989). Molecular Cloning ± doi
  35. (1999). Molecular evidence supports Phytophthora quercina as a distinct species. doi
  36. (1997). Molecular identi®cation of mycorrhizal fungi by direct ampli®cation of microsatellite regions. doi
  37. (1997). Parity of MLEE, RAPD and Ca3 hybridization as ®ngerprinting methods for Candida albicans.
  38. (1999). PAUP: Phylogenetic Analysis using Parsimony. Version 4±0. Sinauer Associates, doi
  39. (1996). PCR-based molecular discrimination of Verticillium chlamydosporium isolates. doi
  40. (1993). PHYLIP (Phylogeny Inference Package). Version 3±5c.
  41. (1995). Phylogenetic relationship among several Leptosphaeria species based on their ribosomal DNA sequences. doi
  42. (1994). Phylogeny of Metarhizium: analysis of ribosomal DNA sequence data. doi
  43. (1997). Production, survival and evaluation of liquid culture-produced inocula of Coniothyrium minitans against Sclerotinia sclerotiorum. doi
  44. (1993). Rapid identi®cation and differentiation of yeasts by DNA and PCR ®ngerprinting.
  45. (1985). Rapid preparation of DNA from ®lamentous fungi. doi
  46. (1997). Revision of Trichoderma sect. Longibrachiatum including related teleomorphs based on analysis of ribosomal DNA internal transcribed spacer sequences. doi
  47. (1990). Screening for sclerotial mycoparasites of Sclerotinia sclerotiorum. doi
  48. (1998). Splash dispersal of Coniothyrium minitans in the glasshouse. doi
  49. (2000). Success in biological control of plant pathogens and nematodes by microorganisms. doi
  50. (1980). The Coelomycetes. Commonwealth Mycological Institute, doi
  51. (1998). The genus Oidiodendron: species delimitation and phylogenetic relationships based on nuclear ribosomal DNA analysis. doi
  52. (2000). The ins and outs of DNA ®ngerprinting the infectious fungi. doi
  53. (1998). The role of soil mesofauna in dispersal of Coniothyrium minitans: mechanisms of transmission. doi
  54. (1996). The use of AFLP ®ngerprinting for the detection of genetic variation in fungi. doi
  55. (1996). TREEVIEW: An application to display phylogenetic trees on personal computers. doi
  56. (1997). Two divergent intragenomic rDNA ITS 2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. doi
  57. (1995). Use of Coniothyrium minitans and Gliocladium virens for biocontrol of Sclerotinia sclerotiorum in glasshouse lettuce. doi
  58. (1993). World distribution of the sclerotial mycoparasite Coniothyrium minitans. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.