Skip to main content
Article thumbnail
Location of Repository

Theoretical model for the formation of caveolae and similar membrane invaginations

By Pierre Sens and Matthew S. Turner

Abstract

We study a physical model for the formation of bud-like invaginations on fluid lipid membranes under tension, and apply this model to caveolae formation. We demonstrate that budding can be driven by membrane-bound proteins, provided that they exert asymmetric forces on the membrane that give rise to bending moments. In particular, caveolae formation does not necessarily require forces to be applied by the cytoskeleton. Our theoretical model is able to explain several features observed experimentally in caveolae, where proteins in the caveolin family are known to play a crucial role in the formation of caveolae buds. These include 1), the formation of caveolae buds with sizes in the 100-nm range and 2), that certain N- and C-termini deletion mutants result in vesicles that are an order-of-magnitude larger. Finally, we discuss the possible origin of the morphological striations that are observed on the surfaces of the caveolae

Topics: QR
Publisher: Biophysical Society
Year: 2004
OAI identifier: oai:wrap.warwick.ac.uk:911

Suggested articles

Citations

  1. (2000). A molecular dissection of caveolin-1 membrane attachment and oligomerization. Two separate regions of the caveolin-1 C-terminal domain mediate membrane binding and oligomer/ oligomer interactions in vivo. doi
  2. (1993). Bending rigidity of SOPC membranes containing cholesterol. doi
  3. (2001). Caveolae and their coat proteins, the caveolins: from electron microscopic novelty to biological launching pad. doi
  4. (2000). Caveolae: uniform structures with multiple functions in signaling, cell growth, and cancer. Exp. Cell Res. doi
  5. (2000). Caveolin-1 regulates shear stress-dependent activation of extracellular signal-regulated kinase. doi
  6. (1992). Caveolin, a protein component of caveolae membrane coats. doi
  7. (2001). Cell surface area regulation and membrane tension.
  8. (2000). Cholesterol and caveolae: structural and functional relationships. doi
  9. (2001). Clathrin-mediated endocytosis: membrane factors pull the trigger. Trends Cell Biol. doi
  10. (1998). Crowded little caves: structure and function of caveolae. doi
  11. (1986). Curvature instability in membranes. doi
  12. (1993). Curvature-induced lateral phase segregation in twocomponent vesicles. doi
  13. (1999). Direct demonstration of the endocytic function of caveolae by a cell-free assay.
  14. (1998). Dynamin at the neck of caveolae mediates their budding to form transport vesicles by GTPdriven fission from the plasma membrane of endothelium. doi
  15. (1990). Entropy-driven tension and bending elasticity in condensed-fluid membranes. doi
  16. (1997). Flexible membranes with anchored polymers. Colloids Surf. doi
  17. (1998). In Search of a New Biomembrane Model. Biologiske Skrifter,
  18. (1996). Inclusions in membranes. doi
  19. (2003). Interactions between proteins bound to biomembranes. doi
  20. (2001). Local entropic effects of polymers grafted to soft interfaces. doi
  21. (1999). Membrane microdomains and caveolae. doi
  22. (1999). Membrane structure of caveolae and isolated caveolin-rich vesicles. doi
  23. (1996). Membranes with anchored polymers at the adsorption transition. doi
  24. (1999). Microphase separation versus the vapor-liquid transition in systems of spherical particles. doi
  25. (1996). Modulation of membrane dynamics and cell motility by membrane tension. Trends Cell Biol. doi
  26. (1998). Mutational analysis of caveolininduced vesicle formation. Expression of caveolin-1 recruits caveolin-2 to caveolae membranes. doi
  27. (1995). Oligomeric structure of caveolin: implications for caveolae membrane organization. doi
  28. (1987). Ordered and curved meso-structures in membranes and amphiphilic films. doi
  29. (2002). Polyme `res greffe ´s sur une membrane: quelques aspects the ´oriques.
  30. (1991). Scaling Concepts in Polymer Physics. doi
  31. Sens and Turner Biophysical Journal 86(4) 2049–20571994. Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease.
  32. (1996). Shape transformations of vesicles with intramembrane domains. doi
  33. (1998). Spontaneous curvature theory of clathrin-coated membranes. doi
  34. (1999). Spontaneous patterning of quantum dots at the air-water interface. doi
  35. (1982). Star-shaped polymers. doi
  36. (1994). Statistical Thermodynamics of Surfaces, Interfaces and Membranes. Perseus, doi
  37. (2000). The shape of polymerdecorated membranes. doi
  38. Theoretical Modeling of Caveolae Formation
  39. (2002). Tilt texture domains on a membrane and chirality induced budding. doi
  40. (1999). Vesicular budding induced by a long and flexible polymer. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.