Location of Repository

In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots

By Stefano Gattolin, Mathias Sorieul, P. R. (Paul R.) Hunter, Roman H. Khonsari and Lorenzo Frigerio

Abstract

Background: Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar\ud compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For\ud several isoforms, the tissue and cell specific pattern of expression are not known.\ud Results: We generated fluorescent protein fusions to the genomic sequences of all members of\ud the Arabidopsis TIP family whose expression is predicted to occur in root tissues (TIP1;1 and 1;2;\ud TIP2;1, 2;2 and 2;3; TIP4;1) and expressed these fusions, both individually and in selected pairwise\ud combinations, in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP\ud distribution varied between different cell layers within the root axis, with extensive co-expression\ud of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose\ud expression overlapped appeared to localise to the tonoplast of the central vacuole, vacuolar bulbs\ud and smaller, uncharacterised structures.\ud Conclusion: We have produced a comprehensive atlas of TIP expression in Arabidopsis roots,\ud which reveals novel expression patterns for not previously studied TIPs

Topics: QK
Publisher: BioMed Central Ltd.
Year: 2009
OAI identifier: oai:wrap.warwick.ac.uk:2337

Suggested articles

Preview

Citations

  1. (2002). A: A complex and mobile structure forms a distinct subregion within the continuous vacuolar membrane in young cotyledons of Arabidopsis. doi
  2. (1998). AF: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. doi
  3. (2003). Benfey PN: A gene expression map of the Arabidopsis root. Science
  4. (1998). Chrispeels MJ: Characterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation. Plant Physiol doi
  5. (1996). Chrispeels MJ: Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site. Plant Cell doi
  6. (1992). Chrispeels MJ: The Expression Pattern of the Tonoplast Intrinsic Protein gamma-TIP in Arabidopsis thaliana Is Correlated with Cell Enlargement. Plant Physiol doi
  7. (2003). de Vries SC: The CUP-SHAPED COTYLEDON3 gene is required for boundary and shoot meristem formation in Arabidopsis. Plant Cell doi
  8. (2008). DG: Multiple vacuoles in plant cells: rule or exception? Traffic doi
  9. (2007). DG: Newly formed vacuoles in root meristems of barley and pea seedlings have characteristics of both protein storage and lytic vacuoles. Plant Physiol doi
  10. (2005). Driouich A: Root border-like cells of Arabidopsis. Microscopical characterization and role in the interaction with rhizobacteria. Plant Physiol doi
  11. (2007). Frigerio L: Fluorescent Reporter Proteins for the Tonoplast and the Vacuolar Lumen Identify a Single Vacuolar Compartment in Arabidopsis Cells. Plant Physiol doi
  12. (2006). Functional aquaporin diversity in plants. Biochim Biophys Acta doi
  13. (2004). HJ: Loss of TIP1;1 aquaporin in Arabidopsis leads to cell and plant death. doi
  14. (2006). JC: A role for caleosin in degradation of oil-body storage lipid during seed germination. doi
  15. (2005). JC: A unique family of proteins associated with internalized membranes in protein storage vacuoles of the Brassicaceae. Plant J doi
  16. (1996). JC: Plant cells contain two functionally distinct vacuolar compartments. Cell doi
  17. (2001). Kjellbom P: The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol doi
  18. (2009). Life with and without AtTIP1;1, an Arabidopsis aquaporin preferentially localized in the apposing tonoplasts of adjacent vacuoles. Plant Mol Biol doi
  19. (2003). Local, efflux-dependent auxin gradients as a common module for plant organ formation. Cell doi
  20. (2005). Maurel C: Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant Physiol doi
  21. (2008). only highly expressed Arabidopsis pollenspecific aquaporins, transport water and urea. FEBS Lett doi
  22. (2007). Plant aquaporins: Novel functions and regulation properties. doi
  23. Plant Cell Vacuoles doi
  24. (2000). PM: pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol
  25. (1998). Rogers JC: deltaTonoplast intrinsic protein defines unique plant vacuole functions.
  26. (1993). Scheres B: Cellular organisation of the Arabidopsis thaliana root. Development doi
  27. (1999). Tonoplast intrinsic protein isoforms as markers for vacuolar functions. Plant Cell doi
  28. (2008). TP: The effects of the loss of TIP1;1 and TIP1;2 aquaporins in Arabidopsis thaliana. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.