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High sensitivity of C. elegans vulval precursor cells to the dose of posterior Wnts.

By Jean-Baptiste Pénigault and Marie-Anne Félix

Abstract

International audienceCell competence is a key developmental property. The Caenorhabditis elegans vulval competence group consists of P(3-8).p, six cells aligned along the antero-posterior axis in a wide central body region. The six cells are not equal in their competence: 1) P3.p quits the competence group in half of the individuals; 2) the posterior cells P7.p and P8.p are less competent than central vulval precursor cells. Competence to adopt a vulval fate is controlled by expression of the HOM-C gene lin-39, and maintained through Wnt signals that are secreted from the tail in a long-range gradient. Here we quantify the LIN-39 protein profile in vulval precursor cells of early L2 stage larvae, prior to P3.p fusion and inductive signaling. We show that LIN-39 levels are very low in P3.p and P4.p, peak in P5.p and progressively decrease until P8.p. This unexpectedly centered profile arises independently from the gonad. Posterior Wnt signaling reduces LIN-39 level in the posterior cells by activating the next-posterior HOM-C gene, mab-5. On the anterior side, P3.p and P4.p competence and division are sensitive to the already low LIN-39 and Wnt doses; most dramatically, each of the cwn-1/Wnt and egl-20/Wnt genes show haplo-insufficience for P3.p fate. In contrast to previous results, we find that these Wnts maintain P3.p and P4.p competence without affecting their LIN-39 level. The centered vulval competence profile is thus under the control of the posterior Wnts and of cross-regulation of three HOM-C genes and prepatterns the later induction of vulval fates

Topics: Cell competence level, C. elegans vulva, Wnt gradient, Hox, Haploinsufficiency, MESH : Animals, MESH : Body Patterning, MESH : Signal Transduction, MESH : Stem Cells, MESH : Vulva, MESH : Wnt Proteins, MESH : Caenorhabditis elegans, MESH : Caenorhabditis elegans Proteins, MESH : Cell Division, MESH : Female, MESH : Gonads, MESH : Larva, MESH : Models, Biological, MESH : Protein Binding, [ SDV.BDD ] Life Sciences [q-bio]/Development Biology
Publisher: Elsevier
Year: 2011
DOI identifier: 10.1016/j.ydbio.2011.06.006
OAI identifier: oai:HAL:hal-00639271v1
Provided by: Hal-Diderot
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