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Sequence variation in the haemagglutinin-neuraminidase gene of human parainfluenza virus type 3 isolates in the UK

By K. R. Collins and A. J. (Andrew J.) Easton

Abstract

The sequence variation in a 934 base-pair region of the gene encoding the haemagglutinin-neuraminidase of five human parainfluenza virus type 3 (HPIV3) isolates was determined together with that of a prototype UK strain. All of the clinical isolates were from the Manchester area of the UK and were obtained in 1990. 1991 and 1993. The gene segment was amplified by the polymerase chain reaction using HPIVB-specific oligonucleotide primers. The nucleotide homology of the strains was high, around 99% and specific differences in the UK sequences when compared with that of the US prototype strain were identified. In addition, a number of isolate-specific differences were seen. No correlation was detected between the observed nucleotide mutations and the year of isolation, which supports the hypothesis that HPIV3 shows cocirculation of a heterogeneous population of viruses rather than varying with time in a linear fashion. However, the data suggested that geographically-defined genetic lineages of HPIV3 may exist

Topics: QR355
Publisher: Cambridge University Press
Year: 1995
OAI identifier: oai:wrap.warwick.ac.uk:2676

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  1. (1994). Analysis ofrespiratory syncitial virus strain variation in successive epidemics in one city.
  2. (1990). Antibody responises of humanis and nonhuman primates to individual antigenic sites of the hemagglutinin-neuraminidase and fusion gleoproteins after primary infection or reinfection with parainfluenza type 3 virus.
  3. (1987). Antigenic analysis of human and bovine parainfluenza virus type 3 strains with monoclonal antibodies. doi
  4. (1985). Antigenic variation in the hemagglutininneuraminidase protein of human parainfluenza type 3 virus. Virology doi
  5. (1990). Antigenic variation of human and bovine parainfluenza virus type 3 strains. doi
  6. (1987). Cloning and nucleotide sequence of newcastle disease virus hemagglutinin-neuraminidase mRNA: Identification of a putative sialic acid binding site. Virology doi
  7. (1980). Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing. doi
  8. (1988). CLUSTAL: A package for performing multiple sequence alignment on a microcomputer. Gene doi
  9. (1989). Epidemiology of parainfluenza virus type 3 in England and Wales over a ten-year period. Epidemiol Infect doi
  10. (1991). Epidemiology of paramyxoviridae. In: doi
  11. (1991). function, and intracellular processing ofthe glycoproteins of paramyxoviridae. In: doi
  12. (1992). Genetic variation of human parainfluenza virus type 1 haemagglutinin-neuraminidase: Analysis of 12 clinical isolates. doi
  13. (1986). Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein.
  14. (1994). Human parainfluenza virus type 1 evolution combines cocirculation of strains and development of geographically restricted lineages. doi
  15. (1988). Influenza B virus evolution - co-circulating lineages and comparison of evolutionary pattern with those of influenza A and influenza C viruses. Virology doi
  16. (1989). Molecular cloning, a laboratory manual, 2nd ed. doi
  17. (1986). Monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. Virology doi
  18. (1985). Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates. Virology doi
  19. (1988). Nucleotide and deduced amino acid sequence of hemagglutinin-neuraminidase genes of human type 3 parainfluenza viruses isolated from 1957 to 1983. Virology doi
  20. (1984). Parainfluenza virus type 3: Seasonality and risk of infection and reinfection in young children. doi
  21. (1989). Primer-directed enzymatic amplification of-dna with a thermostable dna polymerase. Science doi
  22. (1992). Sequence analysis of the 22K, SH and G genes of turkey rhinotracheitis and their intergenic regions reveals a gene order different from that ofother pneumoviruses. .1 Gen Virol doi
  23. (1993). Sequence and structure alignment ofparamyxovirus hemagglutinin-neuraminidase with influenza virus neuraminidase.
  24. (1986). The antigenicity and evolution ofinfluenza HI hemagglutinin, from 1950-1957 and 1977-1983: 2 pathways from one gene. Xrirology

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