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Immunohistochemical studies on supporting and chromaffin cells in the Chinese hamster adrenal medulla in the prehibernating season

By Takao Suzuki, タカオ スズキ and Li Xiang Wan


publisher青森市国立情報学研究所の「学術雑誌公開支援事業」により電子化されました。The adrenal medulla of the Chinese hamster, one of the hibernating rodents, was used. Cryostat sections fixed with 4% paraformaldehyde or Zamboni fluid were immuno stained by the labelled streptavidin biotin (LSAB) method using antisera to S-100 protein and phenylethanolamine N-methyltransferase (PNMT). PNMT-immunoreactive cells (adrenaline (A) cells) were observed mainly in central regions, and PNMT-immunonegative cells (noradrenaline (NA) cells) chiefly in peripheral regions of the adrenal medulla. S-100 protein-immunoreactive cells were more numerously and densely distributed in NA- than in A-cell regions. In NA-cell regions, S-100 protein-labelled cells were present almost evenly in both marginal and inner portions of the parenchyma, and immunoreactive elements of supporting cells formed the coarse network. In A-cell regions, S-100-positive cells were scarcely found. This close cellular association of supporting cells with NA cells in the adrenal medulla may reflect the biological and functional characteristics in the prehibernating season

Topics: adrenal medulla, supporting cell, Chinese hamster
Publisher: Aomori kenritsu hoken daigaku kiyo henshu iinkai
Year: 2002
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