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Altered structure and expression of c-myc in Feline T-cell tumours

By Douglas Forrest, David Onions, Gillian Lees and James C. Neil

Abstract

The c-myc gene is rearranged in a subset of feline T-cell lymphosarcomas. Detailed mapping of c-myc rearrangements showed that some result from feline leukaemia virus (FeLV) proviral integration within or upstream of c-myc, but one case involves a complex 3′ alteration and amplification which is apparently not directly virus-induced. S1 nuclease mapping of RNA from normal cells using c-myc probes revealed two presumptive 5′ ends, each corresponding to a promoter-like sequence (P1 and P2), and a major 3′ discontinuity which mapped to the 3′-most of two possible polyadenylation signals. Analysis of RNA from a series of tumours revealed different modes of c-myc expression. All tumours produced P1 and P2 transcripts with apparently normal structure except for one case where an insertion in intron 1 displaced axon 1 sequences. The abundance ratio of Pl/P2 transcripts varied considerably and was high in tumours which carry a rearrangement adjacent to c-myc, but some other T-cell tumours with no apparent myc alteration displayed an equally high ratio. However, a consistent feature was the lack of detectable RNA from normal c-myc alleles in tumours which express a rearranged c-myc allele or a transduced FeLV v-myc gene. We suggest that this may prove to be a useful indicator of the presence of an oncogenically active myc gene, whether this is a rearranged c-myc or transduced c-myc sequence

Publisher: Elsevier
Year: 1987
OAI identifier: oai:eprints.gla.ac.uk:123624
Provided by: Enlighten
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