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Transcription factor EBF1 is essential for the maintenance of B cell identity and prevention of alternative fates in committed cells

By R. Nechanitzky, D. Akbas, S. Scherer, I. Györy, T. Hoyler, S. Ramamoorthy, A. Diefenbach and R. Grosschedl

Abstract

The transcription factors EBF1 and Pax5 have been linked to activation of the B cell lineage program and irreversible loss of alternative lineage potential (commitment), respectively. Here we conditionally deleted Ebf1 in committed pro-B cells after transfer into alymphoid mice. We found that those cells converted into innate lymphoid cells (ILCs) and T cells with variable-diversity-joining (VDJ) rearrangements of loci encoding both B cell and T cell antigen receptors. As intermediates in lineage conversion, Ebf1-deficient CD19<sup>+</sup> cells expressing Pax5 and transcriptional regulators of the ILC and T cell fates were detectable. In particular, genes encoding the transcription factors Id2 and TCF-1 were bound and repressed by EBF1. Thus, both EBF1 and Pax5 are required for B lineage commitment by repressing distinct and common determinants of alternative cell fates

Year: 2013
OAI identifier: oai:escidoc.org:escidoc:2348852
Provided by: MPG.PuRe
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