Skip to main content
Article thumbnail
Location of Repository

Effects of long-term soluble vs. insoluble dietary fiber intake on high-fat diet-induced obesity in C57BL/6J mice

By Frank Isken, Susanne Klaus, Martin Osterhoff, Andreas F. H. Pfeiffer and Martin O. Weickert


Although most of the proposed beneficial effects of fiber consumption have been attributed to viscous and gel-forming properties of soluble fiber, it is mainly insoluble cereal fiber and whole grains that are strongly associated with reduced diabetes risk in prospective cohort studies, indicating that other unknown mechanisms are likely to be involved.\ud \ud We performed a long-term study investigating potential protective effects of adding soluble guar fiber (10% w/w) vs. insoluble cereal fiber (10% w/w) to an isoenergetic and macronutrient matched high-fat diet in obesity-prone C57BL/6J mice. After 45 weeks, mice fed soluble vs. insoluble fiber showed both significantly increased body weight (41.8±3.0 vs. 33.6±1.5 g, P=.03) and elevated markers of insulin resistance. In mice fed soluble fiber, energy loss via the feces was significantly lower and colonic fermentation with production of short chain fatty acids (SCFA) was markedly increased. Gene expression analysis in white adipose tissue showed significantly increased levels of the fatty acid target G-protein coupled receptor-40 in soluble fiber-fed mice. Liver gene expression in the insoluble fiber group showed a pattern consistent with increased fatty acid oxidation. The present results show that soluble vs insoluble dietary fiber added to a high-fat, Western-style diet differently affected body weight and estimates of insulin sensitivity in obesity-prone mice. Soluble fiber intake with increased SCFA production significantly contributed to digested energy, thereby potentially outweighing the well known short-term beneficial effects of soluble fiber consumption.\ud \u

Topics: QL
Publisher: Elsevier Inc.
Year: 2010
OAI identifier:

Suggested articles


  1. (2005). Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology doi
  2. (2007). and germ intake and risk of type 2 diabetes: a prospective cohort study and systematic review. PLoS Med doi
  3. Boeing H Fiber and magnesium intake and incidence of type 2 diabetes: a prospective study and metaanalysis. doi
  4. Carbohydrate fermentation decreases hepatic glucose output in healthy subjects. doi
  5. (2006). Cereal fiber improves whole-body insulin sensitivity in overweight and obese women. Diabetes Care doi
  6. Chemical inhibition of citrate metabolism alters glucose metabolism in mice. doi
  7. Coactivation of Foxa2 through Pgc-1beta promotes liver fatty acid oxidation and triglyceride/VLDL secretion. doi
  8. (1997). Collier GR Dietary guar gum improves insulin sensitivity in streptozotocin-induced diabetic rats.
  9. (2005). Dietary glycemic index and glycemic load, carbohydrate and fiber intake, and measures of insulin sensitivity, secretion, and adiposity in the Insulin Resistance Atherosclerosis Study. Diabetes Care doi
  10. (2002). Effect of wheat bran on glycemic control and risk factors for cardiovascular disease in type 2 diabetes. Diabetes Care doi
  11. Effect of whole grains on insulin sensitivity in overweight hyperinsulinemic adults.
  12. (2001). Ernst E Guar gum for body weight reduction: meta-analysis of randomized trials. doi
  13. Frataxin deficiency in pancreatic islets causes diabetes due to loss of beta cell mass. doi
  14. Frayn KN Insulin-sensitizing effects of dietary resistant starch and effects on skeletal muscle and adipose tissue metabolism.
  15. Frayn KN Prior short-term consumption of resistant starch enhances postprandial insulin sensitivity in healthy subjects.
  16. Honey CR Guar gum consumption in adolescent and adult rats: short- and long-term metabolic effects. doi
  17. Impact of cereal fibre on glucoseregulating factors. doi
  18. Jayawickreme CK The G-protein-coupled receptor 40 family (GPR40-GPR43) and its role in nutrient sensing. doi
  19. Jesri A Effect of a high-fiber diet vs a fiber-supplemented diet on C-reactive protein level.
  20. Mechanisms underlying the resistance to diet-induced obesity in germ-free mice. doi
  21. Mouse GPR40 heterologously expressed in Xenopus oocytes is activated by short-, medium-, and long-chain fatty acids. doi
  22. Pfeiffer AF Metabolic effects of dietary fiber consumption and prevention of diabetes.
  23. Roberts SB Dietary fiber and weight regulation. doi
  24. Role of Foxa-2 in adipocyte metabolism and differentiation. doi
  25. Soluble dietary fibre improves insulin sensitivity by increasing muscle GLUT-4 content in stroke-prone spontaneously hypertensive rats. doi
  26. The contribution of the large intestine to energy supplies in man.
  27. The FFA receptor GPR40 links hyperinsulinemia, hepatic steatosis, and impaired glucose homeostasis in mouse. doi
  28. Viscous and nonviscous fibres, nonabsorbable and low glycaemic index carbohydrates, blood lipids and coronary heart disease. doi
  29. Wheat-fibre-induced changes of postprandial peptide YY and ghrelin responses are not associated with acute alterations of satiety. doi
  30. Zarzuelo A Effects of dietary fibers on disturbances clustered in the metabolic syndrome. doi

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.