Location of Repository

Transcriptomics of liver and muscle in Holstein cows genetically divergent for fertility highlight differences in nutrient partitioning and inflammation processes

By Bruce Moran, Sean B Cummins, Christopher J. Creevey and Stephen T. Butler

Abstract

peer-reviewedBM and SC were funded by the Teagasc Walsh Fellowship Scheme. This project was supported by Teagasc (RMIS 6075) and DAFM grant 13/S/528 led by SB. CJC was funded under the Science Foundation Ireland (SFI) Stokes lecturer scheme (07/SK/B1236A).Background The transition between pregnancy and lactation is a major physiological change for dairy cows. Complex systemic and local processes involving regulation of energy balance, galactopoiesis, utilisation of body reserves, insulin resistance, resumption of oestrous cyclicity and involution of the uterus can affect animal productivity and hence farm profitability. Here we used an established Holstein dairy cow model of fertility that displayed genetic and phenotypic divergence in calving interval. Cows had similar genetic merit for milk production traits, but either very good genetic merit for fertility traits (‘Fert+’; n = 8) or very poor genetic merit for fertility traits (‘Fert-’; n = 8). We used RNA sequencing to investigate gene expression profiles in both liver and muscle tissue biopsies at three distinct time-points: late pregnancy, early lactation and mid lactation (-18, 1 and 147 days relative to parturition, respectively). Results We found 807 and 815 unique genes to be differentially expressed in at least one time-point in liver and muscle respectively, of which 79 % and 83 % were only found in a single time-point; 40 and 41 genes were found differentially expressed at every time-point indicating possible systemic or chronic dysregulation. Functional annotation of all differentially expressed genes highlighted two physiological processes that were impacted at every time-point in the study, These were immune and inflammation, and metabolic, lipid and carbohydrate-binding. Conclusion These pathways have previously been identified by other researchers. We show that several specific genes which are differentially regulated, including IGF-1, might impact dairy fertility. We postulate that an increased burden of reactive oxidation species, coupled with a chronic inflammatory state, might reduce dairy cow fertility in our model.Teagasc Walsh Fellowship ProgrammeDepartment of Agriculture, Food and the MarineScience Foundation Irelan

Topics: Gene expression, Dairy cow fertility, Liver, muscle, Lactation
Publisher: Biomed Central
Year: 2016
DOI identifier: 10.1186/s12864-016-2938-1
OAI identifier: oai:t-stor.teagasc.ie:11019/1069
Provided by: T-Stór
Journal:

Suggested articles

Preview


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.