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Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine

By Babu Ramanathan, Chit Laa Poh, Kristin Kirk, William John Hannan McBride, John Aaskov and Lara Grollo

Abstract

Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine

Publisher: Public Library of Science
Year: 2016
DOI identifier: 10.1371/journal.pone.0155900
OAI identifier: oai:vtl.cc.swin.edu.au:swin:49053
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