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Zearalenone determination in high performance liquid chromatography : a new standardization method for commercial swine rations

By Janaína Susan Cover, Mário Lettieri Teixeira, Luís Flávio Oliveira Souza and Alexandre Meneghello Fuentefria

Abstract

El propósito de este artículo es ofrecer un nuevo método de cromatografía líquida de alta resolución (CLAR) para evaluar las raciones especiales para cerdos (REC) contaminado con zearalenona (ZEA). Después de la extracción y purificación de ZEA, las muestras se eluyeron con acetonitrilo, metanol y agua del sistema disolvente. Los resultados indican que el método pro¬puesto demostró ser rápido y eficaz para la detección y cuantificación de ZEA en REC, ya que sus indicadores se presentan capaces de recuperación de 102,62%, además de ofrece una excelente precisión, con un coeficiente de variación de 0,9992. Por otra parte, también se propone una prueba de control biológico de hongos micotoxige¬nic aislados y mantenidos en el laboratorio. La prueba se realizó con la levadura killer Trichosporum insectorum CBS10422 contra Fusarium sp y Aspergillus flavus, mostrando eficaces sólo contra Aspergillus.The aim of this work was to offer a new method of high performance liquid chromatography (HPLC) to evaluate commercial swine rations (CSR) contaminated by zearalenone (ZEA). After ZEA extraction and purification from CSR, the samples were eluted with acetonitrile, methanol and water solvent system. The results indicated that the proposed method showed to be rapid and efficient for the detection and quantification of ZEA in CSR, since its recovery was 102.62%, it offered excellent precision with a coefficient of variation of 0.9992. Furthermore, it is also proposed a as a biocontrol assay for micotoxigenic fungi isolated and maintained in the laboratory. The test was performed with the killer yeast Trichosporum insectorum CBS 10422 against Fusarium sp and Aspergillus flavus, which demonstrated to be effective against the latter

Topics: Zearalenona, CLAR, Control biológico, Micotoxinas, Zearalenone, Cromatografia liquida de alta eficiencia, HPLC, Biocontrol
Year: 2013
OAI identifier: oai:agregador.ibict.br.PC_UFRGS:oai:www.lume.ufrgs.br:10183/71658
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