Location of Repository

Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil

By Aline Almeida Uehara, Efigenia L. T. Amorin, Maria de Fatima Ferreira, Cláudia Ferreira de Andrade, Maysa Beatriz Mandetta Clementino, Ivano de Filippis, Felipe P. G. Neves, Tatiana de C. A. Pinto, Lucia M. Teixeira, Marcia Giambiagi Demarval and Sergio E. L. Fracalanzza

Abstract

Despite the rapid spread of antibiotic resistance among gonococci worldwide, limited reports are available from Brazilian locations. In the present study, 25 quinolone-resistant Neisseria gonorrhoeae (QRNG) strains isolated in Rio de Janeiro, Brazil, were characterized by phenotypic and molecular methods, including analysis of mutations in the gyrA and parC genes. They represented 16.5% of the N. gonorrhoeae isolates obtained during a survey performed from 2006 to 2010. A trend for increasing resistance to ciprofloxacin was observed in the period investigated. The most prevalent pattern of mutation observed among QRNG isolates, Ser-91 to Phe and Asp-95 to Gly in gyrA and Ser-87 to Arg in parC, was detected in 40% of the isolates exhibiting MICs ranging from 4 to >32 mug/ml. Rare types of mutations were found in the gyrA gene (Gln-102 to His [12%] and Asp-95 to Tyr [4%]) and in the parC gene (Ser-88 to Thr [4%]). The genetic relationship of the QRNG isolates, evaluated by pulsed-field gel electrophoresis, suggested that the increase in the frequencies of the QRNG isolates in Rio de Janeiro, Brazil, may have arisen as a result of simultaneous spread of two clonal groups. The results also indicate that fluoroquinolones may no longer be used as first line antibiotics for the treatment of gonorrhea in Rio de Janeiro, and that programs for antimicrobial susceptibility surveillance of N. gonorrhoeae should also be implemented in other regions of Brazil

Topics: Neisseria gonorrhoeae, Quinolonas, Testes de Sensibilidade Microbiana, Mutação de Sentido Incorreto, Análise por Conglomerados, Tipagem Molecular, Neisseria gonorrhoeae, Quinolones, Microbial Sensitivity Tests, Mutation, Missense, Cluster Analysis, Molecular Typing, Neisseria gonorrhoeae, Quinolonas, Testes de Sensibilidade Microbiana, Mutação de Sentido Incorreto, Análise por Conglomerados, Tipagem Molecular
Publisher: American Society for Microbiology
Year: 2011
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/11029
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.rcaap.pt/detail.jsp... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.