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Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment

By Marlon G. Veloso de Santana, Patrícia C. C Neves, Juliana Ribeiro dos Santos, Noemia S Lima, Alexandre A. C. dos Santos, David I Watkins, Ricardo Galler and Myrna C Ronaldo


We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness

Topics: Febre Amarela, Yellow Fever 17D viru, Viral vector, Genetic stability, SIV gag IRES, SUV Gag, Recombinant virus, Fiebre Amarilla, Ciências Biológicas
Publisher: Elsevier
Year: 2014
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