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Ultrastructural comparison of external morphology of immature stages of Lutzomyia (Nyssomyia) intermedia and Lutzomyia (Nyssomyia) whitmani (Diptera: Psychodidae), vectors of cutaneous leishmaniasis, by scanning electron microscopy.

By Ana Cristina Bahia, Nágila Francinete Costa Secundino, José Carlos Miranda, Deboraci Brito Prates, Ana Paula Almeida de Souza, Fernando de Freitas Fernandes, Aldina Maria Prado Barral and Paulo Filemon Paolucci Pimenta

Abstract

Lutzomyia (Nyssomyia) intermedia (Lutz & Neiva 1912) and Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho 1939) (Diptera: Psychodidae) are vectors of American cutaneous leishmaniasis in several endemic regions of Brazil. We analyzed the external morphological aspects of the immature stages of these two vectors by using scanning electron microscopy. In general, the larval stages of the two species are morphologically similar, although some differences were noted. Detailed examination of the eggs of both species revealed similar exchorionic ornamentations of unconnected parallel ridges. The larval head capsules are well deÞned, heavily sclerotized, and bear prominent chewing mouthparts. The abdominal segments are easily recognized by the presence of prolegs on their ventral surfaces. The morphology of the anal lobe on the terminal abdominal segment differs between the two species. We found the following three types of sensillae inserted on the antennae: 1) clavate basiconic; 2) small, blunt coeloconic; and 3) multipourous clavate coleoconic. In addition; Þve subtypes of trichoid sensillae were found on the larval body: 1) long, 2) short, 3) curved long, 4) brush-like, and 5) weakly brush-like. The caudal Þlaments located on the last abdominal segment were recognized as long trichoid sensillae. We observed pores on the surface of the clavate coelonic sensillae and on the caudal Þlaments that presumably function as chemoreceptors. The larvae of the two species show similarities in the lobular-form antennae of L1 larvae, which changes to digitiform in second instar (L2), L3, and L4. This study demonstrated that the external surface of the eggs and larvae of Lu. intermedia and Lu. whitmani are morphologically similar, but they can be distinguished by details in the microanatomy observed by scanning electron microscop

Topics: Lutzomyi Intermedia, Lutzomyi whitmani, Immature stages, Scanning electron microscopy, Leishmaniose Cutânea/transmissão, Psychodidae/ultraestrutura, Animais, Insetos Vetores, Larva/ultraestrutura, Microscopia Eletrônica de Varredura, Óvulo/ultraestrutura
Publisher: Entomological Society of America
Year: 2007
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/9232
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