Location of Repository

Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization

By Haendel Gonçalves Nogueira Oliveira Busatti, Ricardo José Alves, Karla Graziela Santana dos Anjos Fonseca, Frederico Ferreira Gil, Marcia C Cury, Marcos André Vannier dos Santos and Maria Aparecida Gomes

Abstract

The chemotherapeutic agents used for the treatment of giardiasis are often associated with adverse side effects and are refractory cases, due to the development of resistant parasites. Therefore the search for new drugs is required. We have previously reported the giardicidal effects of metronidazole (MTZ) and its analogues (MTZ-Ms, MTZ-Br, MTZ-N(3), and MTZ-I) on the trophozoites of Giardia lamblia. Now we evaluated the activity of some giardicidal MTZ analogues in experimental infections in gerbils and its effects on the morphology and ultrastructural organization of Giardia. The giardicidal activity in experimental infections showed ED(50) values significantly lower for MTZ-I and MTZ-Br when compared to MTZ. Transmission electron microscopy was employed to approach the mechanism(s) of action of MTZ analogues upon the protozoan. MTZ analogues were more active than MTZ in changing significantly the morphology and ultrastructure of the parasite. The analogues affected parasite cell vesicle trafficking, autophagy, and triggered differentiation into cysts. These results coupled with the excellent giardicidal activity and lower toxicity demonstrate that these nitroimidazole derivates may be important therapeutic alternatives for combating giardiasis. In addition, our results suggest a therapeutic advantage in obtaining synthetic metronidazole analogues for screening of activities against other infectious agents

Topics: Giardicidal activity, Metronidazole analogues, Parasite load, Electron microscopy, Antiprotozoários/farmacologia, Giardia lamblia/efeitos de drogas, Giardíase/parasitologia, Metronidazol/análogos & derivados, Análise de Variância, Animais, Linhagem Celular, Gerbillinae, Giardia lamblia/citologia, Giardia lamblia/ultraestrutura, Concentração Inibidora 50, Metronidazol/farmacologia, Microscopia Eletrônica de Transmissão, Carga Parasitária, Trofozoítos/citologia, Trofozoítos/efeitos de droga
Publisher: Elsevier Inc
Year: 2013
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/8711
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.rcaap.pt/detail.jsp... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.