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Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood

By Tiffany Weinkopff, Camila Indiani de Oliveira, Augusto Marcelino Pedreira de Carvalho, Yazmin Hauyon LaTorre, Aline de Couto Muniz, José Carlos Miranda, Aldina Maria Prado Barral and Fabienne Tacchini Cottier

Abstract

During a blood meal, Lutzomyia intermedia sand flies transmit Leishmania braziliensis, a parasite causing tegumentary leishmaniasis. In experimental leishmaniasis, pre-exposure to saliva of most blood-feeding sand flies results in parasite establishment in absence of any skin damages in mice challenged with dermotropic Leishmania species together with saliva. In contrast, pre-immunization with Lu. intermedia salivary gland sonicate (SGS) results in enhanced skin inflammatory exacerbation upon co-inoculation of Lu. intermedia SGS and L. braziliensis. These data highlight potential unique features of both L. braziliensis and Lu. intermedia. In this study, we investigated the genes modulated by Lu. intermedia SGS immunization to understand their potential impact on the subsequent cutaneous immune response following inoculation of both SGS and L. braziliensis. The cellular recruitment and global gene expression profile was analyzed in mice repeatedly inoculated or not with Lu. intermedia. Microarray gene analysis revealed the upregulation of a distinct set of IFN-inducible genes, an immune signature not seen to the same extent in control animals. Of note this INF-inducible gene set was not induced in SGS pre-immunized mice subsequently co-inoculated with SGS and L. braziliensis. These data suggest the parasite prevented the upregulation of this Lu. intermedia saliva-related immune signature. The presence of these IFN-inducible genes was further analyzed in peripheral blood mononuclear cells (PBMCs) sampled from uninfected human individuals living in a L. braziliensis-endemic region of Brazil thus regularly exposed to Lu. intermedia bites. PBMCs were cultured in presence or absence of Lu. intermedia SGS. Using qRT-PCR we established that the IFN-inducible genes induced in the skin of SGS pre-immunized mice, were also upregulated by SGS in PBMCs from human individuals regularly exposed to Lu. intermedia bites, but not in PBMCs of control subjects. These data demonstrate that repeated exposure to Lu. intermedia SGS induces the expression of potentially host-protective IFN-inducible genes

Topics: Interações Hospedeiro-Parasita, Leishmania braziliensis/imunologia, Psychodidae/imunologia, Saliva/imunologia, Animais, Brasil, Células Cultivadas, Feminino, Perfilação da Expressão Gênica, Humanos, Interferons/imunologia, Leucócitos Mononucleares/imunologia, Camundongos, Camundongos Endogâmicos BALB C, Reação em Cadeia da Polimerase em Tempo Real
Publisher: Public Library of Science
Year: 2014
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/8407
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