Skip to main content
Article thumbnail
Location of Repository

O uso da talidomida como terapia adjuvante na leptospirose experimental

By Luciane Marieta Soares

Abstract

A leptospirose ?? uma zoonose de import??ncia global, causada por leptospiras patog??nicas. Seu tratamento ?? limitado quando iniciado ap??s quatro dias do surgimento de sintomas, portanto, novas terapias adjuvantes s??o necess??rias. Objetivo. Testar a droga imunomoduladora talidomida como terapia adjuvante ?? ampicilina no modelo de tratamento tardio da leptospirose experimental em hamsters. M??todos. 60 hamsters foram infectados via intraperitoneal por Leptospirainterrogans cepa L1-130, e foram separados em grupos: nenhum tratamento (NONE), talidomida (TAL), ampicilina (AMP) e ambos (AMP-TAL). A talidomida foi administrada via sonda orog??strica na dosagem de 50 mg/Kg dilu??da em ??leo de linha??a (2ml/Kg) por tr??s dias. J?? a ampicilina por via intramuscular: 100mg/Kg/bid por seis dias. Foram realizados dois desenhos experimentais. Experimento 1: o tratamento foi iniciado 48h ap??s o in??cio dos sinais cl??nicos da doen??a. Experimento 2: o tratamento foi iniciado imediatamente ap??s a detec????o do primeiro ??bito entre os animais infectados. Resultados. Experimento1: todos os hamsters dos grupos AMP e AMP-TAL sobreviveram (n=8) enquanto todos os animais dos grupos NONE (n=6) e TAL (n=8) morreram. Os hamsters dos grupos AMP e AMP-TAL n??o mostraram les??es renais, pulmonares ou hep??ticas. Baixa carga de leptospiras foi detectada nos ??rg??os alvo. Experimento 2: o desfecho letal foi observado em 6/6 hamsters no grupo NONE, 8/8 do grupo TAL, 6/8 do AMP e 6/8 do AMP-TAL. A talidomida usada como adjuvante n??o prolongou a sobrevida dos animais tratados com ampicilina. No entanto, o grupo TAL apresentou sobrevida mais longa que o grupo que n??o recebeu tratamento algum. Os animais tratados apresentaram maior frequ??ncia de destrabecula????o de hepat??citos. Conclus??o: Esse estudo abre novas perspectivas no desenvolvimento de um tratamento tardio na leptospirose, contudo observou-se que a talidomida utilizada como terapia adjuvante n??o teve impacto na sobrevida de hamsters como modelo de in??cio tardio de terapia antimicrobiana.Leptospirosis is a zoonosis of global importance, caused by pathogenic leptospira. His treatment is limited when started after four days of onset of symptoms, increasing the risk of morbidity and mortality, so new adjuvant therapies are needed.Objectives.To test the immunomodulatory drug, thalidomide, as an adjuvant therapy to antibiotics in experimental leptospirosis. Methods. Hamsters were infected by Leptospirainterrogans strain L1-130, and groups were assigned based on no treatment (NONE), thalidomide only (TAL), ampicillin only (AMP) or both (AMP-TAL). Thalidomide was administered via a gastric tube: 50 mg/kg in linseed oil and 2 ml/kg for three days. Ampicillin was administered intramuscularly at the rate of 100 mg/kg/bid for six days. Treatment was started two days after the onset of symptoms (experiment 1) and immediately after detection of the first death (experiment 2). Results. Experiment 1: all hamsters from the groups AMP and AMP-TAL survived (n=8), while all hamsters from groups NONE (n=6) and TAL (n=8) died. The AMP and the AMP-TAL groups showed no renal, lung or liver pathology and absent or very low leptospiral burden in target organs. Experiment 2: lethal outcome was observed in 6/6 hamsters in the NONE group, 8/8 in the TAL group, and 6/8 in the AMP group and the AMP-TAL group. Thalidomide showed no survival benefit when compared to hamsters treated with ampicillin alone. The TAL group, however, had a longer interval between infection and death when compared to the NONE group. Treated animals had a higher frequency of liver plate disarray. The TAL, AMP and AMP-TAL groups had very low tissue leptospiral counts. Conclusion: This study may open new perspectives to assist the development of a delayed treatment in leptospirosis, however it was observed that thalidomide used as adjuvant therapy had no impact on the survival of hamsters as late onset of antimicrobial therapy model

Topics: Leptospirose, Leptospira, Talidomida, Modelos animais, Leptospirosis, Leptospira, Thalidomide, Animal disease models
Year: 2013
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/7524
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.rcaap.pt/detail.jsp... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.