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Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor

By Charles B Shoemaker, Hema Ramachandran, Abraham Landa, Mitermayer Galvão dos Reis and Lincoln D Stein

Abstract

The complete coding DNA for a Schistosoma mansoni homologue of the epidermal growth factor receptor (SER) was characterized from cDNA clones obtained by homology to the tyrosine kinase domain of erbB. The DNA sequence predicts a 200-kDa translation product that contains a secretory leader, a cysteine-rich extracellular domain, a hydrophobic transmembrane sequence, and an intracellular tyrosine kinase domain. The SER transcript is present in cercariae and adult schistosomes. In addition to SER transcripts, schistosomes produce at least 3 variant transcripts encoding truncated SER products that include the secretory leader and a small portion of the extracellular domain followed by short sequences of unrelated, Cterminal amino acids. Based on these sequences, 2 of the variant mRNAs (class 2 and 5) appear to encode soluble, secreted proteins while one (class 4) encodes an SER variant protein with a hydrophobic C-terminus that may serve as a membrane anchor. Class 2 SER variant transcripts are present at levels comparable to SER transcripts in adult worms but are not detected in cercariae. Class 4 and 5 SER variant transcripts are also found within adult worms but at lower levels. Genomic cloning and characterization demonstrate that the variant SER transcripts arise through alternative splicing of the SER gen

Topics: Schistosome, Schistosoma mansoni, EGF, EGF receptor, Alternative splicing, Membrane protein, Processamento de RNA, Receptor do Fator de Crescimento Epidérmico/genética, Schistosoma mansoni/genética, Sequência de Aminoácidos, Animais, Sequência de Bases, Clonagem Molecular, DNA/genética, Proteínas de Helminto/genética, Dados de Sequência Molecular, RNA Mensageiro/genética, Homologia de Sequência do Ácido Nucleico, Transcrição Genética
Publisher: Elsevier Science Publishers B.V
Year: 1992
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/7581
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