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Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice

By Salvador Iborra, Nuria Parody, Daniel R Ab??nades, Pedro Bonay, Deboraci Prates, Fernanda O Novais, Manoel Barral-Netto, Carlos Alonso and Manuel Soto

Abstract

In the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice.We show that BALB/c mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides (CpG-ODN) were protected against the development of dermal pathology and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent specific-IFN-g response mediated mainly by CD4?? T cell, albeit a minor contribution of CD8?? T cells cannot be ruled out. Induction of Th1 responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain protection was associated with a LRP specific IFN-g production in lymph nodes draining the challenge site. We believe that these evolutionary conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan-Leishmania vaccine. 2008 Elsevier Masson SAS. All rights reserve

Topics: Leishmania, BALB/C mice, C57BL/6 mice, Th1/Th2 immune responses;, Ribosomal proteins, Vaccines, Leishmania major/imunologia, Vacinas contra Leishmaniose, Leishmaniose Cut??nea/preven????o & controle, Oligodesoxirribonucleot??deos/imunologia, Prote??nas Riboss??micas/imunologia, Animais, Feminino, Imunoglobulina G/metabolismo, Interferon gama/metabolismo, Interleucina-10/metabolismo, Interleucina-4/metabolismo, Leishmaniose Cut??nea/imunologia, Camundongos, Camundongos Endog??micos BALB C, Camundongos Endog??micos C57BL, Oligodesoxirribonucleot??deos/gen??tica, Oligodesoxirribonucleot??deos/metabolismo, Prote??nas de Protozo??rios/gen??tica, Prote??nas de Protozo??rios/imunologia, Prote??nas de Protozo??rios/metabolismo, Prote??nas Riboss??micas/gen??tica, Prote??nas Riboss??micas/metabolismo
Publisher: Elsevier Masson SAS
Year: 2008
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/7408
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