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HEV, TTV and GBV-C/HGV markers in patients with acute viral hepatitis

By André de Castro Lyra, J. R. R Pinho, Luciano Kalabric Silva, L Sousa, C. P Saraceni, Eduardo Lorens Braga, J. E Pereira, Maria Alice Sant’Anna Zarife, Mitermayer Galvão dos Reis, Luiz Guilherme Costa Lyra, L. C. da Silva and Flair José Carrilho

Abstract

The aim of the present study was to evaluate the prevalence of HEV, TTV and GBV-C/GBV-C/HGV in patients with acute viral hepatitis A, B and non-A-C. We evaluated sera of 94 patients from a sentinel program who had acute hepatitis A (N = 40), B (N = 42) and non-A-C (N = 12); 71 blood donors served as controls. IgM and anti-HEV IgG antibodies were detected by enzyme immunoassay using commercial kits. TTV and GBV-C/HGV were detected by nested PCR; genotyping was done by sequencing and phylogenetic analysis. Anti-HEV IgG was present in 38, 10 and 17 percent of patients with hepatitis A, B and non-A-C. Four patients with hepatitis A and 1 with non-A-C hepatitis also had anti-HEV IgM detected in serum. TTV was detected in 21 percent of patients with acute hepatitis and in 31 percent of donors. GBV-C/HGV was detected in 9 percent of patients with hepatitis, and in 10 percent of donors. We found TTV isolates of genotypes 1, 2, 3, and 4 and GBV-C/HGV isolates of genotypes 1 and 2. Mean aminotransferase levels were lower in patients who were TTV or GBV-C/HGV positive. In conclusion, the detection of anti-HEV IgM in some acute hepatitis A cases suggests co-infection with HEV and hepatitis E could be the etiology of a few cases of sporadic non-A-C hepatitis in Salvador, Brazil. TTV genotype 1, 2, 3 and 4 isolates and GBV-C/HGV genotype 1 and 2 strains are frequent in the studied population. TTV and GBV-C/HGV infection does not appear to have a role in the etiology of acute hepatitis

Topics: Acute hepatitis, Hepatitis E, TTV, GBV-C/HGV, Genotype, Brazil, Hepatite Viral Humana/virologia, Anticorpos Anti-Hepatite/sangue, Vírus da Hepatite E/imunologia, Torque teno virus/imunologia, Vírus GB C/imunologia, -Hepatite Viral Humana/diagnóstico, Hepatite Viral Humana/epidemiologia, Vírus da Hepatite E/genética, Torque teno virus/genética, Vírus GB C/genética, Marcadores Biológicos, Vigilância de Evento Sentinela, Estudos de Casos e Controles, Imunoglobulina G/sangue, Imunoglobulina M/sangue, Reação em Cadeia da Polimerase, Genótipo, Índice de Gravidade de Doença, Doença Aguda, Prevalência, Brasil/epidemiologia, Humanos, Masculino, Feminino
Year: 2005
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/5950
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