Humoral immune response of patients bitten by the snake Bothrops erythromelas

Abstract

Introducao: Envenenamentos of??dicos consistem problema de sa??de p??blica em ??reas rurais de pa??ses tropicais e subtropicais, mas pouco sabe-se sobre a resposta imune apresentada pelos indiv??duos picados, por isso a avalia????o da produ????o de IgM por pacientes picados por Bothrops erythromelas identificando a efic??cia do tratamento nesse tipo de envenenamento. Metodos: O veneno de Bothrops erythromelas foi submetido a eletroforese e transferido para nitrocelulose, seguindo incuba????o com soro de pacientes. Resultados: Foi observada prote??na de 38KDa antes e 24 horas ap??s o tratamento. Conclusoes: Os resultados sugerem que essa prote??na poderia ser utilizada como marcador para indiv??duos envenenados pela serpente Bothrops erythromelas.Introduction: Snake envenomings are a health problem in rural ??reas of tropical and subtropical countries, but little is known regarding the immune response presented by bitten individuals. The IgM production of patients bitten by Bothrops erythromelas snake was analyzed to identify the effectiveness of treatment in this type of envenomation. Methods: Bothrops erythromelas venom was submitted to electrophoresis and transferred to a nitrocellulose sheet, following incubation with patients??? sera. Results: A 38 KDa protein was detected before and 24 h after therapy. Conclusions: The result suggests that this protein could be used as a marker for individuals envenomed by Bothrops. erythromelas