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Caracteriza????o morfol??gica, bioquimica e molecular de Vickermania Itaguaiensis N. Gen, SP (Protozoa, Kinetoplastida)

By Renato da Silva Junior

Abstract

A fam??lia Trypanosomatidae inclui parasitas de uma grande variedade de vertebrados, invertebrados (principalmente insetos), plantas e algumas esp??cies de protozo??rios, sendo, depois do nemat??ides, os de maior distribui????o de hospedeiros na natureza. No presente trabalho, um novo isolado foi obtido por coprocultivo de trato intestinal de Leptoglossus stigma Herb, 1784 (Hemiptera, Coreidae), capturado no munic??pio de Itagua??/RJ. O isolado original e tr??s clones (obtidos por citometria de fluxo) foram depositados na "Cole????o de Flagelados do Laborat??rio de Transmissores de Leishmanioses." Um clone foi caracterizado por diversas abordagens em compara????o com esp??cies de refer??ncia de diferentes g??neros. Foi analisado o crescimento em meio de cultivo em intervalos de 24 h, entre 48-144 h, a diferencia????o celular e a morfometria dos principais est??gios evolutivos encontrados. A an??lise de isoenzimas foi realizada utilizando-se os seguintes sistemas: GPI, PGM, 6PGDH, HK, ACON, MPI, FUM, IDH, MDH e ACON. RAPD-PCR foi realizado utilizando-se 6 iniciadores, conjuntamente com outros tripanosomat??deos. Os dados destas an??lises foram processados numericamente e submetidos ?? an??lise computacional utilizando-se o coeficiente de associa????o Jaccard e o algoritmo de agrupamento UPGMA. Realizou-se seq??enciamento do amplicon do gene de SSU rRNA e o fragmento analisado foi alinhado com vinte e uma seq??encias depositadas no GenBank, gerando uma ??rvore filogen??tica resultante do pareamento. O conjunto de resultados deste trabalho sugere que a amostra obtida constitui nova esp??cie, pertencendo a um novo g??nero, nomeada Vickermania itaguaiensis.The Trypanosomatidae family includes parasites of a wide variety of vertebrates, invertebrates (mainly insects), plants and some species of protozoa, and after the nematodes, the highest distribution of hosts in nature. In this study, an isolate was obtained by coproculture from Leptoglossus stigma Herb, 1784 (Hemiptera, Coreidae) intestinal tract, captured in the city of Itagua??/RJ. The original isolate and clones (obtained by flow cytometry) were deposited in the "Cole????o de Flagelados do Laborat??rio de Transmissores de Leishmaniose". One clone was characterized by several approaches in comparison with the reference species of different genus. Growth was analyzed in culture medium at 24 h intervals between 48-144 h, cell differentiation and morphometric parameters of the main evolutionary stages matches. The isoenzyme analysis was performed using the following systems: GPI, PGM, 6PGDH, HK, ACON, MPI, FUM, IDH, MDH and ACON. RAPD-PCR was performed using 6 primers, together with other trypanosomatids. The analysis of these data were numerically processed and submitted to computer analysis using the Jaccard association coefficient and UPGMA clustering algorithm. We carried out sequencing of the amplicon from SSU rRNA gene and the fragment analyzed was aligned with twenty-one sequences deposited in GenBank, generating a phylogenetic tree resulting from the pairing. The result set of this work suggests that the sample obtained represents a new species belonging to a new genus, named Vickermania itaguaiensis

Topics: Vickermania Itaguaiensis, Caracteriza????o Morfol??gica, Trypanosomatina, DNA de Protozo??rio, Kinetoplastida, Flagelos/gen??tica, Isoenzimas /an??lise, C??lulas Clonais, Biologia Computacional, An??lise Biol??gica, Bioqu??mica, Bases de Dados de ??cidos Nucleicos/utiliza????o
Year: 2011
OAI identifier: oai:agregador.ibict.br.RI_FIOCRUZ:oai:localhost:icict/5528
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