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Microbes and Infection

By Cristina T. Fonseca, Edécio Cunha Neto, Anna C. Goldberg, Jorge Kalil, Amélia R. de Jesus, Edgard M. Carvalho, Rodrigo Correa Oliveira and Sérgio C. Oliveira

Abstract

Trabalho completo: acesso restrito, p. 204–212he development of a defined anti-schistosomiasis vaccine would contribute to the current control strategy mainly because immunization provides long-lasting immunity to the disease. Sm14, one of the six Schistosoma mansoni antigens selected by WHO as a candidate to compose a subunit vaccine against schistosomiasis, has been associated with resistance to S. mansoni infection in human beings and is able to induce protection in the murine model. To identify human T cell epitopes in Sm14, we used the TEPITOPE algorithm to select peptides that would most likely bind to several HLA-DR molecules. In this study, three Sm14 epitopes were selected and produced as synthetic peptides. Human T cell responses from schistosomiasis patients living in endemic areas in Brazil were determined by proliferation assay and IL-5 and IFN-γ measurements. Differential peptide recognition and cytokine production in response to Sm14 epitopes were observed in individuals resistant to S. mansoni infection versus susceptible individuals. Sm14(32-48) and Sm14(53-69) peptides were preferentially recognized by peripheral blood mononuclear cells (PBMCs) of S. mansoni-resistant individuals, and Sm14(53-69) induced significant production of IFN-γ. Additionally, Sm14(32-48) and Sm14(53-69) were “promiscuous” peptides, since they were able to induce cellular immune responses in individuals carrying 10 and 8, respectively, of the 11 HLA-DR molecules expressed in the studied population. Among Sm14 synthetic peptides tested in this study, we identified Sm14(32-48) and Sm14(53-69) as promising candidates to compose an anti-schistosomiasis vaccine, since they seem to be related to resistance to human schistosomiasis

Topics: Vaccine, Human T cells, Synthetic peptides, Schistosoma mansoni, Sm14
Publisher: Elsevier
Year: 2012
OAI identifier: oai:agregador.ibict.br.RI_UFBA:oai:192.168.11:11:ri/6607
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