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Anisotropy techniques in study of cytoplasm

By Kateřina Sýkorová


The main goal of this thesis was to compare experiments using time-resolved anisotropy and steady-state anisotropy for measuring in bacteria strain Cupriavidus necator. Fluorescent probe for anisotropy imaging was chosen BCECF_AM, which is derivate of fluorescein. Using experiment in system glycerol/water with fluorescein, anisotropy has been verified and calculated molecular hydrodynamic volume of a single fluorescein molecule, which approximately corresponded with real value. By using fluorescence imaging anisotropy microscopy, images and values of average anisotropy in cells were taken. Images of living cells (bacteria) of CN H16 and mutant CN PHB-4 showed differences, mainly in the uniformity of the inside environment

Topics: fluorescenční mikroskopie; fluorescein; steady-state anisotropy.; steady-state anizotropie.; fluorescence microscopy; fluorescenční sondy; Anizotropie fluorescence; Anisotropy fluorescence; fluorescence probe
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Year: 2016
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