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Use of molecular-genetic methods for research of colorectal carcinoma

By Tereza Janáková


The aim of this bachelor work is to carry out statistical analysis of mutations in KRAS, BRAF and PIK3CA genes. Introductory part is focused on colorectal carcinoma in general. In following part of the work, we discuss genetics of tumors, description of KRAS, BRAF and PIK3CA genes, molecular-genetic analytical methods, widely used in genetics, especially PCR. Further detection of mutations is described. In the next part of the work, we propose a method for detection of mutations in KRAS, BRAF and PIK3CA genes, which consist in using PCR and commercial kits. The following chapter "Results of Analysis of mutations" contains detailed grafical presentation of mutations. Frequencies of mutations were compared with published data and with data from laboratories in Pilsen and Prague. In the end of the bachalor work, there is summary of results and their possible benefit

Topics: cílená biologická léčba; KRAS gene; Kolorektální karcinom; Targeted Biologic Therapy; gen BRAF; PCR; PIK3CA gene; detekce mutací; gen PIK3CA; Colorectal carcinoma; detection of mutations; gen KRAS; BRAF gene
Publisher: Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií
Year: 2012
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