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Analysis of cow milk caseins by capillary electrophoresis

By Irena Dvořáková


The diploma thesis is dealing with the determination of caseins in lyophilised samples of milk using the method of capillary electrophoresis (CE). This method was optimised and verified by Ivana Micikova in her diploma thesis in 2009. The theoretical part of the diploma thesis gives information about the chemical compound of cow's milk. Caseins and their usage in the food and non-food industries are described in a separate unit. Impact of both the nutritional and non-nutritional factors on the composition of the milk is discussed further. The description and the principles of the CE and a list of the methods of CE used in the last several years are stated at the end of this part. The experimental part is focused on the preparation of the solutions, process of casein isolation and its analysis. It summarises the setting of the experiment, sampling and sample processing and the composition of the feeding mixtures. The diploma thesis has arisen on the basis of the cooperation of the Institute of Food Science and Biotechnology, the Faculty of Chemistry, Brno University of Technology and the Research Institute for Cattle Breeding, Ltd., Department of Animal Nutrition Physiology in Pohorelice

Topics: use of casein; složení kravského mléka; bovine milk composition; capillary electrophoresis; využití kaseinů; milk caseins determination; dairy cow nutrition; kaseiny v mléce; caseins in milk; výživa dojnic; stanovení kaseinů v mléce; kapilární elektroforéza
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Year: 2010
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