Location of Repository

Inhibition of MicroRNA miR-92a Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia

By Mohammadreza Sharifi, Rasoul Salehi, Yousof Gheisari and Mohammad Kazemi


OBJECTIVE: MicroRNAs (miRNAs) are endogenous non-coding RNAs, 19-25 nucleotides in length involved in posttranscriptional regulation of gene expression in a considerable majority of mRNAs. In many tumors, up- or down-regulation of different miRNAs has been reported. In acute myeloid leukemia up-regulation of miR-92a has been reported in humans in vitro studies. In this study it is mainly aimed to assess the effect of inhibition of miR-92a in viability of acute promyelocytic leukemia (APL).\ud METHODS: We performed inhibition of miR-92a in an acute promyelocytic leukemia (APL) cell line (HL-60) using locked nucleic acid (LNA) antagomir. At different time points after LNA-anti-miR92a transfection, miR-92a quantitation and cell viability were assessed by qRT-real-time-polymerase chain reaction (PCR) and MTT assays. The data was processed using the ANOVA test.\ud RESULTS: Down-regulation of miR-92a in APL cell line (HL-60) by LNA antagomir extensively decreased cell viability in APL. Cell viability gradually decreased over time as the viability of LNA-anti-miR transfected cells was less than 50% of untreated cells at 72 h post-transfection. The difference of cell viability between LNA-anti-miR and control groups was statistically significant (p<0.024).\ud CONCLUSION: Based on our findings, it is concluded that inhibition of miR-92a may represent a potential novel therapeutic approach for treatment of APL

Topics: microRNA, miR-92a, Acute Promyelocytic Leukemia, Locked Nucleic Acid, Internal medicine, RC31-1245, Diseases of the blood and blood-forming organs, RC633-647.5
Publisher: Galenos Yayinevi
Year: 2013
DOI identifier: 10.4274/Tjh.2012.0171
OAI identifier: oai:doaj.org/article:dfc99f7271c64774834d1a595172dc9e
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1308-5263 (external link)
  • https://doaj.org/toc/1300-7777 (external link)
  • http://dx.doi.org/10.4274/Tjh.... (external link)
  • https://doaj.org/article/dfc99... (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.