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Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays

By Michele Campos Alves, Priscila Aparecida de Almeida, Hudson Caetano Polonini, Nádia Rezende Barbosa Raposo, Anderson de Oliveira Ferreira and Marcos Antônio Fernandes Brandão


RP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R² > 0.99), robust, and specific for the determination of epigallocatechin 3-gallate (EGCG), caffeine (CAF), and gallic acid (GA). In a diffusion cell chamber, the release rate of EGCG was 8896.01 µg cm-2. This data showed that EGCG will be able to exert its systemic activity when delivered though the transdermal formulation, due to its good flux rates with the synthetic membrane

Topics: Camellia sinensis, quality control, in vitro drug release, Chemistry, QD1-999
Publisher: Sociedade Brasileira de Química
Year: 2014
DOI identifier: 10.5935/0100-4042.20140117
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