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The electrochemistry of redox enzymes

By Richard George Whitaker


The work presented in this thesis is of two types. Firstly methods for the\ud electrochemical immobilisation of redox enzymes in organic polymers are described.\ud The electrochemical monitoring of the immobilised enzyme reaction by detection of\ud one of the enzyme's products is discussed, and the results obtained for such a system\ud under a variety of experimental conditions are presented.\ud A good understanding of the way in which such a system operates' was\ud obtained by using a specially developed kinetic model., This model is explained\ud fully in the theory chapter of this thesis.\ud A variety of organic polymers were used in the electrochemical\ud immobilisation process, with varying degrees of success. The flexibility of this\ud approach is demonstrated by the use of a variety of immobilisation matrices and also\ud by the development of bienzyme and bilayer devices.\ud The final experimental chapter presents work on the covalent modification of\ud redox enzymes with a variety of, redox centres based. on ferrocene. Although\ud attempts to electrochemically immobilise a modified enzyme were not successful,\ud some interesting information about the kinetic behaviour and stability of a series Of\ud modified enzymes was obtained.\ud An indication of possible work forming an extension to this thesis is given in\ud the final part of this thesis. The electrochemical immobilisation techniques and the\ud procedure for covalently modifying, enzymes using electroactive, groups are\ud relatively recent ideas. Much work remains to be done before a better understanding\ud of these systems is gained

Topics: QD, QP
OAI identifier: oai:wrap.warwick.ac.uk:4235

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  1. (1977). Enýymi Structure and Mechanism,
  2. (1968). Basic Biological Chemistry, doi
  3. (1980). Quantitative Problems in Biochemistry, 6th edn.,
  4. (1988). Am Chem. doi
  5. (1979). Enzymes, 3rd edn.,
  6. Biochemistry, 2nd edn., doi
  7. (1979). Enzymes", 3rd edn.,
  8. (1982). Biological Electrochemistry", doi
  9. (1971). in "Methods in Enzymology",
  10. (1977). in "Electrochemical Studies of Biological Systems", doi
  11. (1987). in "BioSensbrs, Fundam , entals and Applications",
  12. (1984). Charge and Field Effects in Biosystems",
  13. (1985). A cza.. 828
  14. (1986). Hill, in "Electrochemical Sensors for Biomedical Applications",
  15. (1966). Sands, in "Flavins and Flavoproteins".
  16. (1985). in "Implantable Sensors for Closed Loop Prosthetic Systems".
  17. (1983). in "Extended Linear Chain Compounds".
  18. (1986). Faraday Trans.,
  19. (1988). Chem Soc. Faraday Trans.,
  20. (1988). Sensors andActuators,
  21. Reews. Organic Syntheses,
  22. (1978). fethods ofBiochemical Analysis,
  23. (1980). Quantitative Problems in Biochemistry", 6th edn.,
  24. (1979). EHipsometry and Polarised Light",
  25. (1980). Electrochemical Methods, Fundamentals and Applications"'.
  26. (1977). Era)mx Structure and Mechanism",
  27. (1980). Nfoom "Chemistry for the Life Sciences"', 2nd edn.,
  28. (1983). Gartlini, in "Advances in Heterocyclic Chemistry",
  29. KJL Kanazawa a. 6d JA.
  30. (1983). in "Electroanalytical Chemistry".
  31. in "nic Enzymes". doi
  32. (1962). Physicochemical Hydrodynamics",
  33. (1985). Ile Southampton Electrochemistry Group, "Instrumental Methods in Efccuuchcniistry".
  34. (1987). I fillnun and F-F.
  35. (1975). in "FJccmAe Kinetics".
  36. (1979). Principles and Techniques of Practical Biochemistry",
  37. (1975). Offbrdý in "Comprehensible Biochemistry",
  38. (1963). in "Mic Enz)mxs".
  39. (1970). A cta,
  40. (1969). Electrochemistry at Solid'Electrodes",
  41. (1980). Electrochemical Methods, Fundamentals and Applications", doi
  42. (1975). Electrode Kinetics", doi
  43. (1985). Ile Southampton Electrochemistry Group, "Instrumental Methods in Electrochemistry", I st. edn.,

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